KDM4B (N-term) Rabbit Polyclonal Antibody

CAT#: AP52269PU-N

KDM4B (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of JMJD2B Antibody (N-term) in K562 cell line lysates (35ug/lane). JMJD2B (arrow) was detected using the purified Pab.

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  Formalin-fixed and paraffin-embedded human Testis tissue reacted with JMJD2B Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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  Flow cytometric analysis of K562 cells using JMJD2B Antibody (N-term) (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution:

  ELISA: 1/1000.
  Western Blot: 1/1000.
  Flow Cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.

• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 4-31 amino acids from the N-terminal region of human JMJD2B
• Specificity: This antibody recognizes Human JMJD2B / KDM4B (N-term).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: lysine demethylase 4B
• Database Link:
  Entrez Gene 23030 Human
  UniProt ID O94953
• Background: KDM4B / JMJD2B is a jumonji domain containing histone demethylase that specifically demethylates histone H3 at K9 and K36
• Synonyms: JHDM3B, KIAA0876
• Note: Molecular Weight: 121897 Da

Reference Data

• Protein Families: Druggable Genome