Erythrocytes Rabbit Polyclonal Antibody
Specifications
| Product Data | |
| Application | ELISA, ID, IF, R, WB |
|---|---|
| Recommended Dilution | ELISA. Dot blot. Immunoblotting. Indirect Immunofluorescence. Haemolytic titre, at which 50% lysis occurs following mixing of equal volumes of antiserum, Guinea Pig complement (at a 1/60 dilution) and 2% RBC, not less than 1/500 when tested with Bovine Erythrocytes. |
| Reactivity | Bovine |
| Antibody Host | Rabbit |
| Isotype | IgG |
| Clonality | Polyclonal |
| Immunogen | Washed whole Erythrocytes were prepared from fresh blood and used for intravenous immunization. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
| Specificity | Bovine Erythrocytes. To sensitize cells in Haemolytic complement assays and for screening of T and T receptor-bearing lymphocytes. Homologous proteins of different species frequently share antigenic determinants. Cross-reactivity has not been tested. |
| Buffer | PBS, pH 7.2 without preservatives and foreign proteins State: Azide Free State: Lyophilized purified Hyperimmune IgG fraction |
| Reconstitution Method | Restore by adding 1.0 ml of sterile distilled water |
| Concentration | lot specific |
| Purification | Ammonium Sulphate Precipitation and Ion Exchange Chromatography |
| Conjugation | Unconjugated |
| Storage | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Ambient |
| Reference Data | |
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