TIMP3 (175-211) Rabbit Polyclonal Antibody

CAT#: AP32837PU-T

TIMP3 (175-211) rabbit polyclonal antibody, Purified

Product Images

Formalin-Fixed, Paraffin-Embedded Human placenta stained with TIMP3 Antibody.

Specifications

Product Data

• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: Flow Cytometry: 0.5-1 µg/10⁶ cells.
  Immunofluorescence: 1-2 µg/ml.
  Western Blotting: 0.5-1 µg/ml.
  Immunoprecipitation: 1-2 µg/500 µg protein lysate.
  Immunohistochemistry on Formalin-Fixed Paraffin Sections: 1/200-1/400 for 30 minutes at RT.
  Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
  Positive Control: Placenta or breast carcinoma.
• Reactivities: Bovine, Canine, Equine, Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A portion of amino acids 175-211 of Human TIMP3
• Specificity: This antibody recognizes TIMP-3 (Tissue Inhibitor of Metalloproteinase-3).
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Serum
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 30 kDa
• Gene Name: TIMP metallopeptidase inhibitor 3
• Database Link:
  Entrez Gene 7078 Human
  UniProt ID P35625
• Background: TIMP3 (tissue inhibitor of metalloproteinases 3), along with family members TIMP1, TIMP2, and TIMP4, are inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix (ECM). An imbalance between MMPs and the associated TIMPs may play a significant role in the invasive phenotype of malignant tumors.
  TIMP’s inhibit the proteolytic invasiveness of tumor cells and normal placental trophoblast cells. TIMP-3 may be involved in regulating trophoblastic invasion of the uterus as well as in regulating remodeling of the extracellular matrix during the folding of epithelia, and in the formation, branching and expansion of epithelial tubes.
• Synonyms: Protein MIG-5, TIMP-3