P2RX4 Goat Polyclonal Antibody

SKU AP31932PU-N

P2RX4 goat polyclonal antibody, Aff - Purified

Product Data

• Application: ELISA, IF, WB
• Recommended Dilution:

  Peptide ELISA: 1/16000 (Detection limit). ; Western Blot: Approx 48-50kDa band observed in lysates of cell line A549 (calculated MW of 43.4kDa according to Human NP_002551.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Mouse and Rat Kidney and Liver for this particular batch. ; Immunofluorescence: 10 µg/ml. Strong expression of the protein seen in the cytoplasm of A431 cells.; Immunohistochemistry on Paraffin Sections: 5 µg/ml in Paraffin embedded Human Uterus.

• Reactivity: Bovine, Canine, Human, Mouse, Porcine, Rat
• Antibody Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_035156.2.
• Specificity: This antibody recognizes P2rx4
• Buffer: Tris saline, pH~7.3; State: Aff - Purified; State: Liquid purified Ig fraction; Stabilizer: 0.5% BSA; Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Shipping: Blue Ice
• Predicted Protein Size: 43.5 kDa (NP_002551.2)
• Gene Name: purinergic receptor P2X 4
• Database Link:
  Q99571
• Background: The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel with high calcium permeability. The main pharmacological distinction between the members of the purinoceptor family is the relative sensitivity to the antagonists suramin and PPADS. The product of this gene has the lowest sensitivity for these antagonists. Multiple alternatively spliced transcript variants have been identified for this gene although their full-length natures have not been determined.
• Synonyms: P2X purinoceptor 4, ATP receptor, Purinergic receptor

Reference Data