GFAP Chicken Polyclonal Antibody

Snippet: ture. Sections were then treated with primary antibodies diluted in blocking solution overnight at 4 °C on a shaker. Primary antibodies used were mouse NeuN (1:1000, Abcam) and chicken GFAP (1:500,Origene). Sections were then washed 3 times with PBS and treated with secondary antibodies-goat anti-mouse (1:500) and goat anti-chicken (1:500) and incubated at room temperature for 2 h with shakin

Snippet: For further analysis, regions of the cortex and hippocampus were processed using Imaris (Bitplane, Concord, MA) software to quantify cell counts, fluorescence intensity, and surface area ratios. [Iba1 (Wako, 019-19741, 1:300), DAPI (1:1000), GFAP (Origene, AP31806PU-N, 1:1000), NeuN (abcam, ab104225, 1:500), and ThioS (1% in 50% ethanol)]

Snippet: s, and HTT-exon1-Q72-His (cloned from pPGK HTT-exon1-Q25, HTT-exon1-Q72) (Jeong et al, 2011);pCI-neo EGFP-HA (cloned from pCI-neo Fluc-EGFP) (Gupta et al, 2011);pCMV6-Entry HDGF-Myc-Flag (RC204148;OriGene);and cytHDGF-Myc-Flag. To generate cytHDGF, 12 point mutations were introduced into the two nuclear localization signals of HDGF by gene synthesis (Kishima et al, 2002), and a nuclear export

Snippet: X34 (0.04% in 40% ethanol), GFAP (Origene, AP31806PU-N, 1:1,000),