Neurofibromin (NF1) Rabbit Polyclonal Antibody

CAT#: AP31762PU-N

Neurofibromin (NF1) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of extracts from HepG2 cells, using NF1 Antibody. The lane on the right is treated with the synthesized peptide.

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  Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Immunofluorescence analysis of HepG2 cells, using NF1 Antibody. The picture on the right is treated with the synthesized peptide.

Specifications

Product Data

• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA: 1/20000.
  Immunofluorescence: 1/100 - 1/500.
  Immunohistochemistry on Paraffin Sections.
  Western Blot: 1/500 - 1/1000.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide from Human NF1.
  Epitope: Internal.
• Specificity: This antibody detects endogenous levels of total NF1 protein.
• Formulation: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM Sodium Chloride, 0.02% Sodium Azide and 50% Glycerol
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: neurofibromin 1
• Database Link:
  Entrez Gene 18015 MouseEntrez Gene 24592 RatEntrez Gene 4763 Human
  UniProt ID P21359
• Background: Mutations linked to neurofibromatosis type 1 led to the identification of NF1. NF1 encodes the protein neurofibromin, which appears to be a negative regulator of the ras signal transduction pathway. In addition to type 1 neurofibromatosis, mutations in NF1 can also lead to juvenile myelomonocytic leukemia. Alternatively spliced NF1 mRNA transcripts have been isolated, although their functions, if any, remain unclear.
• Synonyms: NF1,NF-1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: MAPK signaling pathway