ITIH4 (890-903) Goat Polyclonal Antibody

CAT#: AP31103PU-N

ITIH4 (890-903) goat polyclonal antibody, Aff - Purified

Product Images

ITIH4 antibody staining of Human Placenta lysate at 0.03 ug/ml (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Peptide ELISA: Detection Limit: 1/16000.
  Western Blot: 0.03-0.1 µg/ml. In Human Placenta lysates, a band of ~150kDa is detected corresponding to the glycosylated form.
  Immunohistochemistry on Paraffin Sections.
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the C Terminus of the protein sequence according to NP_002209.2 and NP_001159921.1.
• Specificity: This antibody is expected to recognize both reported isoforms (NP_002209.2, NP_001159921.1).
• Formulation: Tris saline, pH~7.3 with 0.02% Sodium Azide and 0.5% BSA
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: inter-alpha-trypsin inhibitor heavy chain family member 4
• Database Link:
  Entrez Gene 3700 Human
  UniProt ID Q14624
• Background: The inter-a-trypsin inhibitor (ITI, IaI) family, a typical and classical example for protein-glycosaminoglycanprotein (PGP) complexes, occurs constitutively in plasma at relatively high concentrations and is a result of alternate combinations of three kinds of heavy chains with a common light chain, the bikunin proteoglycan. Bikunin has two proteinase inhibitor domains and belongs to the Kunitz-type protease inhibitor family; it displays an inhibitory activity against trypsin, leukocyte elastase and plasmin. The heavy chains do not have any protease inhibitory properties but have the capacity to interact in vitro and in vivo with hyaluronic acid and this binding promotes the stability of the extra-cellular matrix. The ITI protein family is suspected of playing a key role in the extra-cellular matrix biology.
• Synonyms: ITI heavy chain H4, IHRP, ITIHL1, PK120, ITI-HC4, Gp120
• Note: Calculated Molecular Weight: 103kDa (NP_002209.2).