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IDH1 Goat Polyclonal Antibody

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SKU
AP31099PU-N
IDH1 goat polyclonal antibody, Aff - Purified
Size:
100 ug
Conjugation:
Unconjugated
  $565.00
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Specifications
Specifications
Product Data
Application ELISA, WB
Recommended Dilution Peptide ELISA: Detection Limit: 1/16000.
Western Blot: 0.3-1 µg/ml. A band of ~38kDa is observed in wildtype lysates of S. cerevisiae (Data kindly provided by F. Reggiiori, University Medical Centre Utrecht, Netherlands).
Reactivity Yeast
Antibody Host Goat
Clonality Polyclonal
Immunogen Peptide with sequence from the Internal region of the protein sequence according to NP_014361.1.
Specificity Recognizes IDH1 (Yeast).
Buffer Tris saline, pH~7.3
State: Aff - Purified
State: Liquid purified Ig fraction
Stabilizer: 0.5% BSA
Preservative: 0.02% Sodium Azide
Concentration lot specific
Purification Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Conjugation Unconjugated
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Database Link
Background Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. IDH1 is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes.
IDH1 contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.
Synonyms Isocitric dehydrogenase, YNL037C
Note Calculated Molecular Weight: 39.3kDa (NP_014361.1).
Reference Data
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Documents
Resources

file-alt Citations

A nuclear-based quality control pathway for non-imported mitochondrial proteins
Shakya, Barbeau, Xiao et al
Elife (2021) 10 DOI: 10.7554/eLife.61230
AI Summary: OriGene’s Anti-Idh1 (goat polyclonal) antibody (Cat # Ap31099PU-N) was used for western blot analysis to detect Idh1 protein levels, contributing to the study’s investigation of mitochondrial protein localization and degradation pathways.
Snippet: Cat # F1804 RRID: AB_262044 IF (1:200) Antibody Anti-H2B (rabbit polyclonal) Active Motif Cat # 39947 RRID: AB_2793403 WB (1:1000) Antibody Anti-Idh1 (goat polyclonal) ORIGENE Cat # Ap31099PU-N WB (1:500) Antibody Anti-Ilv2 (rabbit polyclonal) Dr. Agnieszka Chacinska N/A WB (1:1000), IF (1:200) Antibody Anti-Tom70 (rabbit polyclonal);Antibody Anti-Idh1 (goat polyclonal) ORIGENE Cat # Ap31099PU-N WB (1:500)
FigureFigureFigure
WB IF
Showing 1-1 of 1 papers.
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