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DNase I (DNASE1) (1-252) Rabbit Polyclonal Antibody

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SKU
AP23615PU-N
DNase I (DNASE1) (1-252) rabbit polyclonal antibody, Aff - Purified
Size:
50 ul
Conjugation:
Unconjugated
  $535.00
3 Weeks*
Bulk/Customize

View other "DNase I or NP_005214" antibodies

Specifications
Specifications
Product Data
Application IF, IHC, WB
Recommended Dilution Immunocytochemistry: 1/100 - 1/1000.
Immunohistochemistry on Paraffin Sections: 10 µg/ml.
Western Blot: 1/500 - 1/3000.
Reactivity Canine, Human, Rabbit
Antibody Host Rabbit
Clonality Polyclonal
Immunogen Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1 and 252 of DNase I.
Specificity This antibody recognizes Deoxyribonuclease I (DNASE1) at aa 1-252.
Buffer 0.1 M Tris-glycine, pH 7.0 containing 10% Glycerol and 0.01% Thimerosal
State: Aff - Purified
State: Liquid purfied Ig fraction
Concentration lot specific
Purification Immunoaffinity Chromatography
Conjugation Unconjugated
Storage Store the antibody (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Gene Name deoxyribonuclease I
Database Link
Background Deoxyribonuclease I (EC 3.1.21.1) gene is approximately 3.2 kb long with 9 exons separated by 8 introns. In the form of a bovine pancreatic enzyme preparation, it occupies an important place in the history of protein chemistry and enzymology: it was the first enzyme to be recognized as specific for DNA; it was the first DNase to be crystallized; and it was the first DNase for which a specific protein inhibitor was characterized.
DNase I is a Ca2+ and Mg2+ dependant endonuclease. DNase I is synthesized in the pancreas and stored in zymogen granules. It has been used to reduce the viscosity of cystic fibrosis sputum. A DNase I-like enzyme appears to catalyze the degradation of chromatin to oligo- and mononucleosomes during apoptosis. A recent study has demonstrated an endonuclease with activity and antigenicity indistinguishable from DNase I in thymocytes, cells susceptible to apoptosis. DNase I is an endonuclease that hydrolyzes double-stranded or single stranded DNA preferentially at sites adjacent to pyrimidine nucleotides. The product of hydrolysis is a complex mixture of 5'-phosphate mononucleotides and oligonucleotides. In the presence of Mg ion, DNase I attacks each strand of DNA independently and the cleavage sites are random.
Synonyms DNASE1, DNL1, DRNI, Deoxyribonuclease I, DNase I
Reference Data
Protein Categories Allergies and autoimmune diseases, Intracellular Proteins, Secreated Proteins
Protein Families Druggable Genome, Secreted Protein, Transmembrane
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Documents
Resources

file-alt Citations

Cell-autonomous epithelial activation of AIM2 (absent in melanoma-2) inflammasome by cytoplasmic DNA accumulations in primary Sjögren's syndrome
Vakrakou, Svolaki, Evangelou et al
J. Autoimmun. (2020) 108, 102381 DOI: 10.1016/j.jaut.2019.102381
Cited by 57
AI Summary: OriGene products contributed to this study through the use of the DNase1 shRNA (SR304274) and Human DNase1 cDNA clone (RC208706), which were employed to manipulate DNase1 expression in salivary gland epithelial cells, helping to demonstrate the link between DNase1 deficiency, cytoplasmic DNA accumulation, and AIM2 inflammasome activation in primary Sjögren's syndrome.
Snippet: …Rabbit polyclonal antibodies against human IL-1β (ab2105) and AIM2 (ab93015) were purchased from Abcam, against DNase1 (AP23615PU-N) was from Acris Antibodies and against ASC/PYCARD (N-15R) from Santa Cruz Biotechnology;- 2.1. … Rabbit polyclonal antibodies against human IL-1β (ab2105) and AIM2 (ab93015) were purchased from Abcam, against DNase1 (AP23615PU-N) was from Acris Antibodies and against ASC/PYCARD (N-15R) from Santa Cruz Biotechnology
Showing 1-1 of 1 papers.
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