SSH3BP1 (ABI1) (C-term) Rabbit Polyclonal Antibody
Specifications
| Product Data | |
| Application | IF, IHC, WB |
|---|---|
| Recommended Dilution | Western blot: At 1-2μg/ml with the appropriate system to detect Abi-1 in cells and tissues. Immunohistochemistry on paraffin sections: At 0.5-1μg/ml to detect Abi-1 in formalin fixed and paraffin embedded tissues. Immunohistochemistry on frozen sections: At 0.5-1μg/ml to detect Abi-1 in formalin or acetone fixed tissues. Immunocytochemistry: At 0.5-1μg/ml to detect Abi-1 in acetone fixed cell. Antigen retrieval by Pepsin and Trypsin is required. |
| Reactivity | Human, Mouse, Rat |
| Antibody Host | Rabbit |
| Isotype | IgG |
| Clonality | Polyclonal |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminal of human ABI-1 |
| Specificity | This antibody detects ABI1 / SSH3BP1 (C-term). |
| Buffer | 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3 State: Aff - Purified State: Lyophilized Ig fraction |
| Reconstitution Method | 0.2ml of distilled water will yield a concentration of 500μg/ml. |
| Purification | Immunogen affinity purified |
| Conjugation | Unconjugated |
| Storage | Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Ambient |
| Gene Name | abl interactor 1 |
| Database Link | |
| Background | ABI1 is a human homolog of mouse Abl-interactor-1 (Abi1), mapped on 10p11.2. ABI1 participate in the transduction of signals from Ras to Rac by regulating Rac-specific guanine nucleotide exchange factor (GEF) activities. ABI1 dramatically promoted ABL1-mediated tyrosine phosphorylation of MENA,but not of other substrates. Abi-1 regulates c-Abl-mediated phosphorylation of Mena by interacting with both proteins.ABI1 plays a role in the leukemogenesis by translocating to MLL. |
| Synonyms | Abl interactor 1, Abelson interactor 1, Abi-1, e3B1, Spectrin binding protein, Nap1-binding protein, Abl-binding protein 4, AblBP4 |
| Reference Data | |
| Protein Categories | Intracellular Proteins |
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