APOBEC3G (C-term) Goat Polyclonal Antibody

CAT#: AP23049PU-N

APOBEC3G (C-term) goat polyclonal antibody, Purified

Product Images

Western Blot Image: Antibody (0.5 ug/ml) staining of Daudi lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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  Immunohistochemistry Image: Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Immunohistochemistry Image: Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/16000.
  Immunohistochemistry on Paraffin Sections: 2.5 µg/ml.
  Western Blot: 0.5 - 1.5 µg/ml.
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Synthetic peptide from the C-Terminus of Human APOBEC3G (NP_068594.1).
  Percent identity by BLAST analysis: Human, Baboon (100%); Monkey (92%); Chimpanzee, Gorilla, Gibbon, Marmoset (83%).
• Specificity: This antibody detects APOBEC3G (C-term).
• Formulation: Tris saline, pH~7.3
  State: Purified
  State: Liquid purified Ig fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C to -80°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: apolipoprotein B mRNA editing enzyme catalytic subunit 3G
• Database Link:
  Entrez Gene 60489 Human
  UniProt ID Q9HC16
• Background: The Apoliprotein B mRNA-editing, enzyme-catalytic, polypeptide-like (APOBEC) 3 is a multi-isoform member of the cytidine deaminase family of enzymes that act on monomeric nucleoside and nucleotide substrates. Similar to TRIM5a which targets incoming retroviral capsids, APOBEC3 plays a major role in cellular defense against retroviral infection as at least two isoforms, APOBEC3G and to a lesser extent APOBEC3F, can be incorporated HIV-1 virions and induce hypermutation in the newly synthesized viral DNA and thus destabilize the viral genome. This innate mechanism of retroviral resistance is counteracted by the HIV-1 Vif protein by inducing the ubiquitization and degradation of APOBEC3G; a single amino acid substitution (D128K) blocks APOBEC3G depletion without affecting its inhibitory activity.
• Synonyms: ARP9, CEM15, MDS019, APOBEC-3G, ARCD