AIM2 (C-term) Goat Polyclonal Antibody

CAT#: AP22454PU-N

AIM2 (C-term) goat polyclonal antibody, Aff - Purified

Product Images

AIM2 antibody staining of IFNbeta-treated WI-38 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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  AIM2 antibody staining of paraffin embedded Human Spleen at 3.8 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Peptide ELISA: 1/8000 (Detection Limit).
  Western Blot: 1-3 µg/ml. Approx 50+55kDa bands observed in lysates of Human WI-38 fibroblast after treatment with interferon beta (calculated MW of 39.0kDa according to NP_004824.1).
  The observed molecular weight corresponds to earlier findings in literature with different antibodies (Choubey et al., J. Interferon Cytokine Res. 2010 Jun;30(6):371-80. PMID: 20187776).
  Immunohistochemistry on Paraffin Sections: 4-6 µg/ml. In paraffin embedded Human Spleen shows nucleolar staining in some splenocytes.
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the C-Terminus of the protein sequence according to NP_004824.1.
• Specificity: This antibody detects AIM2 at C-term.
• Formulation: Tris saline, pH~7.3
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: absent in melanoma 2
• Database Link:
  Entrez Gene 9447 Human
  UniProt ID O14862
• Background: AIM2 is a member of the IFI20X /IFI16 family. It plays a putative role in tumorigenic reversion and may control cell proliferation. Interferon gamma induces expression of AIM2.
• Synonyms: PYHIN4