AIM2 (C-term) Goat Polyclonal Antibody
Specifications
| Product Data | |
| Application | ELISA, IHC, WB |
|---|---|
| Recommended Dilution | Peptide ELISA: 1/8000 (Detection Limit). Western Blot: 1-3 µg/ml. Approx 50+55kDa bands observed in lysates of Human WI-38 fibroblast after treatment with interferon beta (calculated MW of 39.0kDa according to NP_004824.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Choubey et al., J. Interferon Cytokine Res. 2010 Jun;30(6):371-80. PMID: 20187776). Immunohistochemistry on Paraffin Sections: 4-6 µg/ml. In paraffin embedded Human Spleen shows nucleolar staining in some splenocytes. |
| Reactivity | Human |
| Antibody Host | Goat |
| Clonality | Polyclonal |
| Immunogen | Peptide with sequence from the C-Terminus of the protein sequence according to NP_004824.1. |
| Specificity | This antibody detects AIM2 at C-term. |
| Buffer | Tris saline, pH~7.3 State: Aff - Purified State: Liquid purified Ig fraction Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide |
| Concentration | lot specific |
| Purification | Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide |
| Conjugation | Unconjugated |
| Storage | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Gene Name | absent in melanoma 2 |
| Database Link | |
| Background | AIM2 is a member of the IFI20X /IFI16 family. It plays a putative role in tumorigenic reversion and may control cell proliferation. Interferon gamma induces expression of AIM2. |
| Synonyms | PYHIN4 |
| Reference Data | |
| Protein Categories | Intracellular Proteins |
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