OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

PTCH1 (C-term) Goat Polyclonal Antibody

Be the first to review this product
SKU
AP22448PU-N
PTCH1 (C-term) goat polyclonal antibody, Aff - Purified
Size:
100 ug
Conjugation:
Unconjugated
  $565.00
5 Days*
Bulk/Customize

View other "PTCH1 or NP_000255" antibodies

Specifications
Specifications
Product Data
Application ELISA, IF, IHC
Recommended Dilution Peptide ELISA: 1/8000 (Detection Limit).
Immunohsitochemistry on Paraffin Sections: 3-6 µg/ml.
Staining of Embedded Human Colon shows textured nuclear staining of the absorptive cells.
Immunofluorescence: 5 µg/ml. Expression of the protein seen in the cytoplasm of HeLa and in the Golgi/membrane of NIH3T3 cells.
Reactivity Human, Mouse, Rat
Antibody Host Goat
Clonality Polyclonal
Immunogen Peptide with sequence from the C Terminus of Human PTCH1 (NP_001077073.1, NP_001077074.1, NP_001077075.1, NP_001077076.1)
Specificity This antibody is expected to recognise all reported isoforms of PTCH1.
Variants (NP_001077073.1; NP_001077074.1; NP_001077075.1; NP_001077076.1) encode the same isoform.
Buffer Tris saline, pH~7.3
State: Aff - Purified
State: Liquid purified Ig fraction
Stabilizer: 0.5% BSA
Preservative: 0.02% Sodium Azide
Concentration lot specific
Purification Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
Conjugation Unconjugated
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Gene Name patched 1
Database Link
Background PTCH (Patched protein homolog 1) is a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). PTCH associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal.PTCH has a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis. PTCH is expressed in the adult brain, lung, liver, heart, placenta, skeletal muscle, pancreas and kidney. It is also expressed in tumor cells but not in normal skin. During development PTCH is found in all major target tissues of sonic hedgehog, such as the ventral neural tube, somites, and tissues surrounding the zone of polarizing activity of the limb bud.
Defects in PTCH are probably the cause of basal cell nevus syndrome also known as Gorlin syndrome or Gorlin-Goltz syndrome.
Synonyms PTCH, PTC1, PTC, Patched 1
Reference Data
Protein Categories Cancer: Brain and CNS, Cancer: Skin, Growth Factors, Intracellular Proteins, Membrane Proteins
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
Protein Pathways Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources

file-alt Citations

Expression of Hedgehog ligand and signal transduction components in mutually distinct isocitrate dehydrogenase mutant glioma cells supports a role for paracrine signaling
Abiria, Williams, Munden et al
J Neurooncol (2014) 119 (2), 243-51 DOI: 10.1007/s11060-014-1481-7
Cited by 7
AI Summary: OriGene products contributed to this paper by providing the GLI1 (TA328224) and PTCH1 (TA327322) antibodies, which were used to detect the expression of Hedgehog pathway components in glioma cells.
FigureFigure
Identification of Hedgehog pathway responsive glioblastomas by isocitrate dehydrogenase mutation
Gerardo Valadez, Grover, Carter et al
Cancer Lett. (2013) 328 (2), 297-306 DOI: 10.1016/j.canlet.2012.10.002
Cited by 24
AI Summary: OriGene’s pCMV6-Entry wild-type IDH1 vector was used as a cDNA template to generate IDH1 point mutations for functional studies in this paper.
Snippet: extraction Point mutations (R132H, R132G, R132S and Y183C) were introduced into the IDH1 open reading frame by PCR site-specific mutagenesis (Quick change II XL Site Directed Mutagenesis Kit, Stratagene) using pCMV6-Entry wild-type IDH1 vector (OriGene) as a cDNA
Showing 1-2 of 2 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products