PKM Sheep Polyclonal Antibody
Specifications
| Product Data | |
| Application | ELISA, ID, IF, IP, R, WB |
|---|---|
| Recommended Dilution | Can be used in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques). Working dilutions: vary widely, but may be between 1/250 and 1/1000. |
| Reactivity | Rabbit |
| Antibody Host | Sheep |
| Isotype | IgG |
| Clonality | Polyclonal |
| Immunogen | Pyruvate kinase isolated and purified from rabbit muscle. Freund’s complete adjuvant is used in the first step of the immunization procedur |
| Specificity | Biotin-conjugated IgG fraction of polyclonal sheep antiserum to pyruvate kinase from rabbit muscle. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition. |
| Buffer | PBS, pH 7.2 without preservatives and foreign proteins Label: Biotin State: Lyophilized hyperimmune IgG fraction Molar radio: Biotin/IgG ~9.1 |
| Reconstitution Method | Restore by adding 1.0 ml of sterile distilled water |
| Concentration | lot specific |
| Purification | Ammonium Sulphate Precipitation and Ion Exchange Chromatography |
| Conjugation | Biotin |
| Storage | Prior to and following reconstitution store the antibody at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Ambient |
| Database Link | |
| Synonyms | PK2, PK3, PKM, CTHBP, M2-PK, THBP1, OIP3, OIP-3, Pyruvate kinase 2/3, Pyruvate kinase M1/M2, Pyruvate kinase muscle |
| Reference Data | |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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