3-beta-HSD Rabbit Polyclonal Antibody
Specifications
| Product Data | |
| Application | ELISA, ID, IF, IP, R, WB |
|---|---|
| Recommended Dilution | This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of choice. Working Dilutions: Non-precipitating antibody-binding techniques: at least 1/500. |
| Reactivity | P. testosteroni |
| Antibody Host | Rabbit |
| Isotype | IgG |
| Clonality | Polyclonal |
| Immunogen | beta-Hydroxysteroid Dehydrogenase isolated and purified from Pseudomonas testosteroni cells. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
| Specificity | beta-Hydroxysteroid Dehydrogenase from Pseudomonas testosteroni cells. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and enzyme inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined. |
| Buffer | PBS, pH 7.2 without preservatives and foreign proteins State: Azide Free State: Lyophilized hyperimmune IgG fraction |
| Reconstitution Method | Restore by adding 1.0 ml of sterile distilled water |
| Concentration | lot specific |
| Purification | Ammonium Sulphate Precipitation and Ion Exchange Chromatography |
| Conjugation | Unconjugated |
| Storage | Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Ambient |
| Database Link | |
| Synonyms | 3-beta-hydroxysteroid dehydrogenase |
| Reference Data | |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Citations
Effects of maternal acrolein exposure during pregnancy on testicular testosterone production in fetal rats
Yang, Zhang, Zhang et al
Mol Med Rep (2017)
Yang, Zhang, Zhang et al
Mol Med Rep (2017)
AI Summary: OriGene’s diaminobenzidine (DAB) was used in this study to visualize signals during immunohistochemical analysis, enabling detection of protein expression changes in fetal rat testes following maternal acrolein exposure.
Snippet: Following a further wash with PBS, the slides were incubated with an anti‐converter‐peroxidase secondary antibody, which was part of the TUNEL kit (Roche Diagnostics) for 30 min at 37 ̊C, and the signal was visualized with diaminobenzidine (DAB;OriGene Technologies, Inc., Beijing, China)
Showing 1-1 of 1 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
