PFKM Sheep Polyclonal Antibody

SKU AP21254AF-N

PFKM sheep polyclonal antibody, Azide Free

Product Data

• Application: ELISA, ID, IF, IP, R, WB
• Recommended Dilution: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of; the customer’s own choice.; Working dilutions in non-precipitating antibody-binding techniques: at least 1/200.
• Reactivity: Rabbit
• Antibody Host: Sheep
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fructose-6-Phosphate Kinase is isolated and purified from Rabbit muscle.; Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The antibody recognizes Fructose-6-Phosphate Kinase from Rabbit muscle.; The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.; Cross-reactivities against enzymes of other sources may occur but have not been determined.
• Buffer: PBS, pH 7.2 without preservatives and foreign proteins; State: Azide Free; State: Lyophilized hyperimmune IgG fraction
• Reconstitution Method: Restore by adding 1.0 ml of sterile distilled water
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer.; If a slight precipitation occurs upon storage, this should be removed by centrifugation.
• Stability: Shelf life: one year from despatch.
• Shipping: Ambient
• Database Link:
  P00511
• Synonyms: Phosphofructokinase-M, Phosphohexokinase, Phosphofructokinase 1, PFK1, PFK-1, PFK-A, PFKX, GSD7

Reference Data