ENO3 Sheep Polyclonal Antibody
Specifications
| Product Data | |
| Application | ELISA, ID, IF, IP, R, WB |
|---|---|
| Recommended Dilution | This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques). Working dilutions in non-precipitating antibody-binding techniques: at least 1/200. |
| Reactivity | Rabbit |
| Antibody Host | Sheep |
| Isotype | IgG |
| Clonality | Polyclonal |
| Immunogen | Enolase isolated and purified from Rabbit muscle. Freund’s complete adjuvant is used in the first step of the immunization procedure |
| Specificity | The antibody recognizes Enolase from Rabbit muscle The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined. |
| Buffer | PBS, pH 7.2 without preservatives and foreign proteins Label: Biotin State: Lyophilized hyperimmune IgG fraction Molar radio: Biotin/IgG ~6.7 |
| Reconstitution Method | Restore by adding 1.0 ml of sterile distilled water |
| Concentration | lot specific |
| Purification | Ammonium Sulphate Precipitation and Ion Exchange Chromatography |
| Conjugation | Biotin |
| Storage | Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation. |
| Stability | Shelf life: one year from despatch |
| Shipping | Ambient |
| Database Link | |
| Synonyms | Muscle-specific enolase, Skeletal muscle enolase, Enolase 3, ENO3, ENOB |
| Reference Data | |
Reviews
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| Product Manuals |
| FAQs |
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Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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