Alpha-amylase Rabbit Polyclonal Antibody

SKU
AP21208AF-N
Alpha-amylase rabbit polyclonal antibody, Azide Free
  $395.00
2 Weeks*
Bulk/Customize
Specifications
Specifications
Product Data
Application ELISA, ID, IF, IP, R, WB
Recommended Dilution This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer’s own choice.
Indirect immunofluorescence.
ELISA.
Dot blot,.
Immunoblotting.
Working Dilutions in non-precipitating antibody-binding techniques: 1/50,000-1/250,000.
Reactivity Bacillus sp.
Antibody Host Rabbit
Isotype IgG
Clonality Polyclonal
Immunogen Alpha-Amylase isolated and purified from Bacillus subtilis.
Freund's complete adjuvant is used in the first step of the immunization procedure.
Specificity The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.
Cross-reactivities against enzymes of other sources may occur but have not been determined.
Buffer PBS, pH 7.2 without preservatives and foreign proteins
State: Azide Free
State: Lyophilized hyperimmune IgG fraction
Reconstitution Method Restore by adding 1.0 ml of sterile distilled water
Concentration lot specific
Purification Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Conjugation Unconjugated
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Shipping Ambient
Database Link
Background Amylase catalyses the hydrolysis of internal 1,4 glucan links in large linear polymers at internal bonds. The enzyme is present in all living organisms with variable enzymatic activity.
Reference Data
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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