PRIM1 Rabbit Polyclonal Antibody

CAT#: AP21164PU-N

PRIM1 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of PRIM1 antibody (Cat.-No.: AP21164PU-N) in extracts from Raw264.7 cells, treated with LPS.

• 

  Immunohistochemistry (IHC) analyzes of PRIM1 antibody (Cat.-No.: AP21164PU-N) in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of PRIM1 protein.
  (region surrounding Lys413)
• Formulation: Phosphate buffered saline (PBS), pH 7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 15 mM sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 50 kDa
• Gene Name: primase (DNA) subunit 1
• Database Link:
  Entrez Gene 19075 MouseEntrez Gene 246327 RatEntrez Gene 5557 Human
  UniProt ID P49642
• Background: The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. PRIM1 is the small, 49 kDa primase subunit.The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the small, 49 kDa primase subunit.
• Synonyms: PRIM1, DNA primase 49 kDa subunit

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: DNA replication, Metabolic pathways, Purine metabolism, Pyrimidine metabolism