Adducin 2 (ADD2) Rabbit Polyclonal Antibody

CAT#: AP20545PU-N

Adducin 2 (ADD2) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of Beta-Adducin Antibody (Cat.-No AP20545PU-N) in extracts from 293 cells.

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  Western blot analysis of Beta-Adducin Antibody (Cat.-No AP20545PU-N) at 1/500 dilution.Lane 1: HEK293T whole cell lysate. Lane 2: Raw264.7 whole cell lysate. Lane 3: Rat spleen tissue lysate.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
  Immunofluorescence: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 470-520 of Human Adducin β.
• Specificity: This antibody detects endogenous levels of Adducin beta protein. (region surrounding Glu497)
• Formulation: PBS, pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~81 kDa
• Gene Name: Homo sapiens adducin 2 (ADD2), transcript variant 1
• Database Link:
  Entrez Gene 11519 MouseEntrez Gene 24171 RatEntrez Gene 119 Human
  UniProt ID P35612
• Background: Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta and gamma). Adducin is a protein associated with the inner leaflet of the plasma membrane and is one of the proteins localized at the spectrin-actin junction of the membrane skeleton. The cortical actin cytoskeletal network is lost during apoptosis and adducins are central in the cortical actin network organization. Adducin alpha is a cytoskeletal protein involved with sodium-pump activity in the renal tubule and is associated with hypertension. The expression of Adducin alpha and Adducin gamma is ubiquitous in contrast to the restricted expression of Adducin beta. Adducin beta is expressed at high levels in brain and hematopoietic tissues, such as bone marrow in humans, and spleen in mice.
• Synonyms: ADDB, Adducin 2