UBE2C (C-term) Goat Polyclonal Antibody

CAT#: AP16056PU-N

UBE2C (C-term) goat polyclonal antibody, Aff - Purified

Product Images

Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showiug cytoplasmic stainiug. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).

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  Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showiug cytoplasmic /Plasma Membrane stainiug. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).

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  Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Specifications

Product Data

• Applications: ELISA, FC, IF, IP, WB
• Recommended Dilution: Peptide ELISA: 1/32000 (Detection Limit).
  Western blot: 1-3 µg/ml. Detects a band of ~20KDa in mitotic (i.e Nocodazole arrested) HeLa whole cell lysates.
  Flow Cytometry analysis of HeLa cells: 10 µg/ml.
  Immunoprecipitation: 20KDa band precipitated from mitotic HeLa whole cell lysates using protein-G dynabeads.
  Immunofluorescence: 10 µg/ml. Strong expression of the protein seen in the cytoplasm of U2OS and MCF7 cells.
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide from C Terminus of the protein sequence according to NP_008950.1; NP_861515.1; NP_861516.1; NP_861517.1; NP_861518.1.
• Specificity: This antibody is expected to recognise all reported isoforms. Variants NP_861517.1 and NP_861518.1 encode the same isoform.
• Formulation: Tris saline, pH~7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
• Conjugation: Unconjugated
• Gene Name: ubiquitin conjugating enzyme E2 C
• Database Link:
  Entrez Gene 11065 Human
  UniProt ID O00762
• Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2C is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for the destruction of mitotic cyclins and for cell cycle progression.
• Synonyms: Ubiquitin-conjugating enzyme E2 C, Ubiquitin-protein ligase C, Ubiquitin carrier protein C