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UBE2C (C-term) Goat Polyclonal Antibody

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SKU
AP16056PU-N
UBE2C (C-term) goat polyclonal antibody, Aff - Purified
Size:
100 ug
Conjugation:
Unconjugated
  $565.00
5 Days*
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View other "UBE2C or NP_008950.1" antibodies

Specifications
Specifications
Product Data
Application ELISA, FC, IF, IP, WB
Recommended Dilution Peptide ELISA: 1/32000 (Detection Limit).
Western blot: 1-3 µg/ml. Detects a band of ~20KDa in mitotic (i.e Nocodazole arrested) HeLa whole cell lysates.
Flow Cytometry analysis of HeLa cells: 10 µg/ml.
Immunoprecipitation: 20KDa band precipitated from mitotic HeLa whole cell lysates using protein-G dynabeads.
Immunofluorescence: 10 µg/ml. Strong expression of the protein seen in the cytoplasm of U2OS and MCF7 cells.
Reactivity Human
Antibody Host Goat
Clonality Polyclonal
Immunogen Peptide from C Terminus of the protein sequence according to NP_008950.1; NP_861515.1; NP_861516.1; NP_861517.1; NP_861518.1.
Specificity This antibody is expected to recognise all reported isoforms. Variants NP_861517.1 and NP_861518.1 encode the same isoform.
Buffer Tris saline, pH~7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer
State: Aff - Purified
State: Liquid purified Ig fraction
Concentration lot specific
Purification Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Conjugation Unconjugated
Shipping Blue Ice
Gene Name ubiquitin conjugating enzyme E2 C
Database Link
Background The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2C is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for the destruction of mitotic cyclins and for cell cycle progression.
Synonyms Ubiquitin-conjugating enzyme E2 C, Ubiquitin-protein ligase C, Ubiquitin carrier protein C
Reference Data
Protein Categories Intracellular Proteins
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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