CD3G (N-term) Rabbit Polyclonal Antibody

CAT#: AP11514PU-N

CD3G (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of CD3G Antibody (N-term) in HL60 cell line lysates (35 ug/lane). CD3G (arrow) was detected using the purified Pab (1:60 dilution).

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  Formalin-fixed and paraffin-embedded human brain tissue reacted with CD3G antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: ELISA: 1/1,000.
  Western blot: 1/1,000.
  Flow Cytometry: 1/10-1/50.
  Immunohistochemistry 1/10-1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 48-76 amino acids from the N-terminal region of human CD3G.
• Specificity: This antibody detects CD3G at N-term.
• Formulation: PBS with 0.09% (W/V) sodium azide
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. Then the antibody is purified by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD3g molecule
• Database Link:
  Entrez Gene 917 Human
  UniProt ID P09693
• Background: CD3G is the CD3-gamma polypeptide, which together with CD3-epsilon, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. Defects in the CD3G gene are associated with T cell immunodeficiency.
• Synonyms: CD3G, T3G
• Note: Molecular weight: 20469 Da