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Plakophilin 2 (PKP2) (C-term) Guinea Pig Polyclonal Antibody

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SKU
AP09554SU-N
Plakophilin 2 (PKP2) (C-term) guinea pig polyclonal antibody, Serum
Size:
100 ul
Conjugation:
Unconjugated
  $635.00
2 Weeks*
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Specifications
Specifications
Product Data
Application IF, IHC, WB
Recommended Dilution Western Blot.
Immunofluorescence Microscopy on Frozen Tissue and Cultured Cells (after Formaldehyde fixation).
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin Sections (after Microwave retrieval treatment).
For better accessibility of the desmosome-associated antigen Frozen sections might be pre-incubated for 5 min in PBS plus 0.2% Triton X-100 prior to antibody application. Longer incubation could result in a loss of nuclear PP2-staining. To prevent wash-out of soluble forms of the antigen, fixation with 2% formaldehyde is recommended.
Working Dilutions: 1/100 for Immunofluorescence Microscopy.
Incubation Time: 1 h at RT, extended with paraffin sections.
Reactivity Bovine, Human, Mouse, Rat
Antibody Host Guinea Pig
Clonality Polyclonal
Immunogen Human synthetic Plakophilin 2 (C-terminal aa 820-837)
Specificity This PP2 antiserum stains specifically desmosomal plaques present in simple and glandular epithelia, the basal layer of certain stratified epithelia, all layers of some stratified epithelia, and desmosome-possessing nonepithelial cells such as myocardium, Purkinje fibers, and dendritic reticulum of lymph node follicles. In all these cells, including a variety of cell types devoid of desmosomes, Plakophilin 2 is highly enriched in the karyoplasm.
Polypeptide Reacting: Plakophilin 2, Mr ~100 kD (SDS-PAGE); MW calculated from aa sequence: 92,750 (pI 9.33) and 97,410 (pI 9.38)
Tested Reactivity on Cultured Cell Lines: MCF-7, PLC.
Buffer State: Serum
State: Liquid stabilized antiserum with 0.09% Sodium Azide and 0.5% BSA
Conjugation Unconjugated
Storage Store the antibody undiluted at 2-8°C.
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Gene Name plakophilin 2
Database Link
Background Plakophilin 2 is a member of the arm-repeat family of proteins which can occur in at least 2 splice forms (2a and 2b). Plakophilin 2 assembles with other proteins to form the desmosomal plaque in simple and glandular epithelia, the basal layer of certain stratified epithelia, all layers of some stratified epithelia, and in nonepithelial desmosome-possessing tissues such as myocardium, Purkinje fibers and dendritic reticulum of lymph node follicles. In all these cells, including a variety of cell types devoid of desmosomes, plakophilin 2 is highly enriched in the karyoplasm.
Synonyms PKP2
Reference Data
Protein Categories Cardiovascular diseases, Intracellular Proteins
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Documents
Resources

file-alt Citations

Cell cycle defects underlie childhood-onset cardiomyopathy associated with Noonan syndrome
Meier, Raj Murthi, Rawat et al
iScience (2022) 25 (1), 103596 DOI: 10.1016/j.isci.2021.103596
Cited by 29
AI Summary: OriGene’s Guinea pig polyclonal anti-Plakophilin 2 antibody (Cat# AP09554SU-N) was used in this study to help characterize myocardial tissue and investigate the cellular mechanisms underlying cardiomyopathy in Noonan syndrome.
Snippet: onin T Abcam Cat# ab92546;RRID: AB_10585593 Human monoclonal anti-Cardiac Troponin T-VioBlue Miltenyi Biotec Cat# 130-120-402;RRID: AB_2783891 Guinea pig polyclonal anti-Plakophilin 2 Origene Cat# AP09554SU-N;RRID: AB_10556427 Mouse monoclonal anti-Ki67 BD Biosciences Cat# 556003;RRID: AB_396287 Mouse monoclonal anti-α-actinin Sigma-Aldrich Cat# A7811;RRID: AB_476
FigureFigureFigure
Showing 1-1 of 1 papers.
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