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GST-Tag Goat Polyclonal Antibody

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SKU
AP09205AP-N
Anti-GST (GOAT) Antibody Alkaline Phosphatase Conjugated, 1 mg
Size:
1 mg
Conjugation:
Unconjugated FITC HRP TRITC
  $1,245.00
3 Days*
Bulk/Customize
Specifications
Specifications
Product Data
Application ELISA, IHC, WB
Recommended Dilution WB: 1:500 - 1:2,000
IHC: 1:200 - 1:1,000
ELISA: 1:1,000 - 1:5,000
Reactivity GST-Tag
Antibody Host Goat
Isotype IgG
Clonality Polyclonal
Immunogen Glutathione-S-Transferase Schistosoma japonicum
Specificity Anti-GST Alkaline Phosphatase Conjugated antibody as prepared from monospecific antiserum by immunoaffinity chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Alkaline Phosphatase, as well as purified and partially purified Glutathione-S-Transferase Schistosoma japonicum.  Cross reactivity against Glutathione-S-Transferase from other sources may occur but has not been specifically determined.
Buffer 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.1% (w/v) Sodium Azide
Concentration 1.0 mg/mL - lot specific
Purification Immunoaffinity chromatography
Conjugation Unconjugated
Storage Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Stability Expiration date is one (1) year from date of receipt.
Shipping Blue Ice
Database Link
Background GST Antibody is specific for the GST affinity tag. Affinity tags re appended to proteins thereby allowing them to be purified from their crude biological source using an affinity technique. Common affinity tags include glutathione-S-transferase (GST), chitin binding protein (CBP), maltose binding protein (MBP), and the poly-Histidine or HIS-tag.
Synonyms goat anti-GST antibody alkaline phosphatase conjugation, alk phos conjugated goat anti-GST antibody, Glutathione-S-Transferase, GST antibody, anti-GST antibody, anti-Glutathione-S-Transferase antibody, ALP, Alk Phos
Note Anti-GST Antibody Alkaline Phosphatase conjugated has been tested by ELISA and western blot and is suitable for immunoblotting, ELISA, immunohistochemistry as well as other antibody based assays using alkaline phosphatase conjugates requiring lot-to-lot consistency. Optimal concentrations in these or other immunoassays should be determined by the researcher.
Reference Data
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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