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L1 / ORF2 Chicken Polyclonal Antibody

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SKU
AP09187PU-N
Anti-L1/ORF2 (CHICKEN) Antibody, 100 ug
Size:
100 ug 25 ul
Conjugation:
Unconjugated
  $870.00
3 Days*
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View other "ORF2 or AAD39215.1" antibodies

Specifications
Specifications
Product Data
Application ELISA, WB
Recommended Dilution WB: 1:2,000-1_10,000
ELISA: 1:10,000
Reactivity Chimpanzee, Human
Antibody Host Chicken
Isotype IgY
Clonality Polyclonal
Immunogen This IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with two synthetic peptides conjugated to keyhole limpet hemocyanin (KLH).  The peptides correspond to regions within the endonuclease domain of L1/ORF2 protein.
Specificity L1/ORF2 Antibody is directed against two regions within the endonuclease domain of L1 ORF2 protein.  This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above.   Reactivity occurs against human L1/ORF2 protein and is useful in determining its presence in various assays.   A BLAST analysis was used to suggest cross reactivity with L1/ORF2 proteins from chimpanzee sources based on 100% homology with the immunizing sequences, and from macaque, fruit fly, cattle, dog, opossum, and rat sources based on 69 - 88% homology with the immunizing sequences.
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01% (w/v) Sodium Azide
Concentration 1.0 mg/ml - lot specific
Purification IgY fraction antibody purified from monospecific Chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer.
Conjugation Unconjugated
Storage Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Stability Expiration date is one (1) year from date of receipt.
Shipping Blue Ice
Database Link
Background This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. The L1 (LINE-1), or Long INterspersed Element , retrotransposon ORF2 is the most common open reading frame in the human genome, present in various forms in many thousands of copies. This large family of proteins includes magnesium dependent endonucleases and a large number of phosphatases involved in intracellular signaling. Both intact ORF1 and ORF2 are absolutely required for autonomous retro-transposition. ORF2 encodes an endonuclease, reverse transcriptase, and zinc knuckle domains. The expression of ORF2 appears to be tightly regulated except in germ line tissues, embryonic tissues, and certain cancers including teratomas, testicular cancers, and leukemias. This antibody is intended for use in studying control of L1 retrotransposons.
Synonyms chicken anti-L1/ORF2 Antibody, chicken anti-L1-ORF2 Antibody, L1 (LINE-1), Long INterspersed Element , retrotransposon ORF2
Note Anti-L1/ORF2 Antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 149 kDa in size corresponding to L1 protein by western blotting in the appropriate cell lysate or extract.
Reference Data
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Documents
Resources

file-alt Citations

Neurofascin regulates the formation of gephyrin clusters and their subsequent translocation to the axon hillock of hippocampal neurons
Burkarth, Kriebel, Kranz et al
Mol Cell Neurosci (2007) 36 (1), 59-70 DOI: 10.1016/j.mcn.2007.06.001
Cited by 44
AI Summary: In this study, OriGene’s neurofascin shRNA (OriGene: TF302431) and neurofascin expression vectors (OriGene: SC117964 and SC117965) were used to manipulate neurofascin levels in hippocampal neurons, enabling the authors to demonstrate neurofascin’s essential role in gephyrin cluster formation and their movement to the axon hillock.
Showing 1-1 of 1 papers.
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