EIF2AK3 (601-1115) Rabbit Polyclonal Antibody
$870.00
3 Days*
Specifications
| Product Data | |
| Application | ELISA, IF, IHC, IP, WB |
|---|---|
| Recommended Dilution | WB: 1:500 – 1:3000 IHC: 1:1,000 IF: User Optimized ELISA: 1:4,000 - 1:20,000 IP: 10-30ul |
| Reactivity | Mouse |
| Antibody Host | Rabbit |
| Isotype | IgG |
| Clonality | Polyclonal |
| Immunogen | This whole rabbit serum was prepared by repeated immunizations with a recombinant fusion protein from amino acids 601-1115 of mouse deltaN PERK. |
| Specificity | This antiserum is directed against PERK and reacts with the PERK from mouse tissues. Reactivity to other species is unknown. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: None Preservative: 0.01% (w/v) Sodium Azide |
| Concentration | 80 mg/mL - lot specific |
| Conjugation | Unconjugated |
| Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Stability | Expiration date is one (1) year from date of receipt. |
| Shipping | Blue Ice |
| Gene Name | eukaryotic translation initiation factor 2 alpha kinase 3 |
| Database Link | |
| Background | The PKR-like endoplasmic reticulum kinase (PERK, also know as Eukaryotic translation initiation factor 2-alpha kinase 3) is a type I transmembrane protein localized to the endoplasmic reticulum (ER). PERK consists of an N-terminal ER luminal domain, a membrane-spanning region, and a cytosolic C-terminal serine/threonine kinase domain (1). The luminal domain of PERK is bound to the ER chaperone GRP78 in unstressed cells (2). PERK activation occurs upon accumulation of misfolded proteins and the ER lumen, which triggers GRP78 dissociation from PERK thereby allowing PERK dimerization and autophosphorylation (3, 4). PERK phosphorylates two established targets: the eukaryotic translation initiation factor 2 alpha (eIF2, (1)) and the Nrf2 transcription factor (5). Phosphorylation of eIF2 results in attenuation of translation initiation (6). The translational block also contributes to cell cycle arrest due to loss of the G1 regulatory protein, cyclin D1 (7). PERK-dependent phosphorylation of Nrf2 promotes transcription of phase II detoxifying enzymes which is critically important for elimination of intracellular reactive oxygen species (8). Thus, while inhibiting new protein synthesis and thereby decreasing the ER protein load PERK simultaneously induces expression of genes that help restore cellular redox homeostasis and promote survival. |
| Synonyms | rabbit anti-PKR-like Endoplasmic Reticulum Kinase Antibody, rabbit anti-PERK Antibody, PKR-like endoplasmic reticulum kinase, PERK, Eukaryotic translation initiation factor 2-alpha kinase 3 |
| Note | This antiserum has been tested for use in western blotting, immunoprecipitation and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect bands approximately 150kDa by western blotting in the appropriate cell lysate or extract. |
| Reference Data | |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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