PPAR gamma (PPARG) (Isoform 1) (255 -268) Rabbit Polyclonal Antibody
PPAR gamma (PPARG) (Isoform 1) (255 -268) rabbit polyclonal antibody, Aff - Purified
|Applications||ELISA, IHC, WB|
|Recommended Dilution||ELISA: 1/8000 - 1/32000.
Immunohistochemistry on Paraffin Sections: 5 µg/ml.
|Reactivity||Canine, Duck, Guinea Pig, Hamster, Human, Macaque, Mink, Mouse, Rabbit, Rat, Squirrel|
|Immunogen||Synthetic Peptide corresponding to Amino acids 255 -268 of human PPAR gamma isoform 1.|
|Formulation||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide as preservative
State: Aff - Purified
State: Liquid purified IgG fraction
|Storage||Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
|Stability||Shelf life: one year from despatch.|
|Background||Since their discovery in the early 1990's, the peroxisome proliferator activated receptors (PPARs) have attracted significant attention. This is primarily because PPARs serve as receptors for two very important classes of drugs: the hypolipidemic fibrates and the insulin sensitizing thiazolidinediones. Peroxisome proliferators are non-genotoxic carcinogens that are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed PPARs. Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Upon binding fatty acids or hypolipidemic drugs, PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate the expression of target genes. There are 3 known subtypes of PPARs: PPAR-alpha, PPAR-delta and PPAR-gamma. Mostly target genes are involved in the catabolism of fatty acids. Conversely, PPAR-gamma is activated by peroxisome proliferators such as prostaglandins, leukotrienes and anti-diabetic thiazolidinediones and affects the expression of genes involved in the storage of the fatty acids. PPAR-gamma may also be involved in adipocyte differentiation. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 through interaction with specific response elements.|