ELF5 Rabbit Polyclonal Antibody

CAT#: AP06762PU-N

ELF5 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of Elf-5 antibody in extracts from Jurkat cells.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunofluorescence: 1/50-1/200.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 182-230 of Human Elf-5.
• Specificity: This antibody detects endogenous levels of Elf-5 protein.
  (region surrounding Trp215)
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 31 kDa
• Gene Name: E74 like ETS transcription factor 5
• Database Link:
  Entrez Gene 13711 MouseEntrez Gene 2001 Human
  UniProt ID Q9UKW6
• Background: Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. This family of genes currently includes Ets-1, Ets-2, Erg-1–3, Elk-1, Elf-1, Elf-5, NERF, PU.1, PEA3, ERM, FEV, ER8l, Fli-1, TEL, Spi-B, ESE-1, ESE-3A, Net, ABT1 and ERF. Members of the Ets gene family exhibit varied patterns of tissue expression, and share a highly conserved carboxy terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. This conserved domain is essential for Ets-1 binding to DNA and is likely to be responsible for the DNA-binding activity of all members of the Ets gene family.
  Elf-5 is a member of the Ets family that may be involved in lung, mammary, prostate and kidney function, and may also play a role in tumorigenesis.
• Synonyms: E74-like factor 5, Elf-5

Reference Data

• Protein Families: Transcription Factors