H3.3A (H3F3A) Rabbit Polyclonal Antibody
Specifications
| Product Data | |
| Application | IF, IHC |
|---|---|
| Recommended Dilution | IHC:1/50-200, IF:1/50-200 |
| Reactivity | Human, Mouse, Rat |
| Antibody Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Synthetic peptide, corresponding to the N-terminal of Human Histone H3.3 |
| Specificity | This antibody detects endogenous levels of Histone H3.3 protein. (region surrounding Lys27) |
| Buffer | Phosphate buffered saline (PBS), pH 7.2. State: Aff - Purified State: Liquid purified Ig fraction Preservative: 0.05% sodium azide |
| Concentration | 1.0 mg/ml |
| Purification | Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) |
| Conjugation | Unconjugated |
| Storage | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Predicted Protein Size | ~ 16 kDa |
| Gene Name | H3 histone, family 3A |
| Database Link | |
| Background | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Histone variants differ in amino acid sequence from their core histone counterparts and in some cases have also been shown to have functional differences to the more typical histones. |
| Synonyms | H3F3A, H3.3A, H3F3, PP781 |
| Reference Data | |
| Protein Categories | Intracellular Proteins |
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