Oct-1 (POU2F1) Rabbit Polyclonal Antibody

CAT#: AP06254PU-N

Oct-1 (POU2F1) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of OCT1/OTF1 antibody in extracts from HeLa cells.

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  Immunohistochemistry (IHC) analyzes of OCT1/OTF1 antibody in paraffin-embedded human lymph node tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 30-80 of Human Oct-1.
• Specificity: This antibody detects endogenous levels of OCT1/OTF1 protein.
  (region surrounding Leu68)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 89 kDa
• Gene Name: POU class 2 homeobox 1
• Database Link:
  Entrez Gene 5451 Human
  UniProt ID P14859
• Background: Organic cation transporters (OCT) are expressed in the plasma membrane of epithelial cells from a wide range of tissues, where they function in the elimination of endogenous amines, cationic drugs and other xenobiotics. The structure of OCTs consists of a 12-transmembrane-domain structure and a large extracellular hydrophilic loop. In humans, OCT1 is primarily expressed in the liver while OCT2 is expressed in the kidney. OCT3 is expressed in the placenta, skeletal muscle, prostate, aorta and liver. OCT3, also known as extraneuronal monoamine transporter, is widely expressed in different regions of the brain including the hippocampus, cerebellum and cerebral cortex. OCT3 mediates the uptake of several neuroactive agents, including dopamine, and may play an important role in the disposition of neurotransmitters and cationic neurotoxins in the brain. The genes encoding human OCT1–3 map to a conserved cluster at chromosome 6q26-q27.
• Synonyms: OTF1, NF-A1, Octamer-binding protein 1, Oct-1, Otf-1