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Bromodeoxyuridine / BrDU Sheep Polyclonal Antibody

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SKU
AP05008PU-N
Bromodeoxyuridine / BrDU sheep polyclonal antibody, Purified
Size:
250 ug
Conjugation:
Unconjugated
  $930.00
5 Days*
Bulk/Customize
Specifications
Specifications
Product Data
Application IHC, IP, WB
Recommended Dilution Western blotting: 25-100 µg/ml.
Immunohistochemistry: 25-100 µg/ml.
Immunoprecipitation: 25-100 µg/ml.
The antibody has been tested using Immunoprecipitation against 5-methyl cytosine (5-MeC) and bromodeoxyuridine (BrdU) or a control (no antigen). At a concentration of 25 µg/ml, this product demonstrates 8-fold higher reactivity with 5-MeC versus BrdU. For best results, use product at 25-100 μg/ml.
Note: Denature DNA sample first so that bases are accessible to anti BrdU antibody.
Antibody Host Sheep
Isotype IgG
Clonality Polyclonal
Immunogen Bromodeoxyuridine conjugated to Helix Pomatia Haemocyanin.
Specificity This antibody recognizes BrdU (Bromodeoxyuridine).
Buffer PBS
State: Purified
State: Liquid purified IgG fraction (0.2µm sterile filtered)
Preservative: 0.08% Sodium Azide
Concentration lot specific
Purification Ammonium Sulfate Precipitation
Conjugation Unconjugated
Storage Upon receipt, store undiluted (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Background Bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU. Previous methods of detecting DNA included the use of 3H-thymidine which would be incorporated into the DNA and could then the DNA could be quantified by autoradiography or scintillation counting. These methods are more difficult and require more cleanup due to the radioactive material. An immunohistochemical assay provides a much simpler and cleaner method for detecting DNA in cells.
Reference Data
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Documents
Resources

file-alt Citations

Stroke-induced progenitor cell proliferation in adult spontaneously hypertensive rat brain: effect of exogenous IGF-1 and GDNF
Dempsey, Sailor, Bowen et al
J Neurochem (2003) 87 (3), 586-97 DOI: 10.1046/j.1471-4159.2003.02022.x
Cited by 180
AI Summary: In this study, OriGene’s anti-DCX antibody (Cat# TA309184) and anti-NeuN antibody (Cat# TA336348) were used to identify and characterize newly formed neurons in rat brain tissue following stroke and growth factor treatment.
Showing 1-1 of 1 papers.
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