Bromodeoxyuridine / BrDU Sheep Polyclonal Antibody

SKU AP05008PU-N

Bromodeoxyuridine / BrDU sheep polyclonal antibody, Purified

Product Data

• Application: IHC, IP, WB
• Recommended Dilution: Western blotting: 25-100 µg/ml.; Immunohistochemistry: 25-100 µg/ml. ; Immunoprecipitation: 25-100 µg/ml.; The antibody has been tested using Immunoprecipitation against 5-methyl cytosine (5-MeC) and bromodeoxyuridine (BrdU) or a control (no antigen). At a concentration of 25 µg/ml, this product demonstrates 8-fold higher reactivity with 5-MeC versus BrdU. For best results, use product at 25-100 μg/ml. ; Note: Denature DNA sample first so that bases are accessible to anti BrdU antibody.
• Antibody Host: Sheep
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Bromodeoxyuridine conjugated to Helix Pomatia Haemocyanin.
• Specificity: This antibody recognizes BrdU (Bromodeoxyuridine).
• Buffer: PBS; State: Purified; State: Liquid purified IgG fraction (0.2µm sterile filtered); Preservative: 0.08% Sodium Azide
• Concentration: lot specific
• Purification: Ammonium Sulfate Precipitation
• Conjugation: Unconjugated
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Background: Bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU. Previous methods of detecting DNA included the use of [3H]-thymidine which would be incorporated into the DNA and could then the DNA could be quantified by autoradiography or scintillation counting. These methods are more difficult and require more cleanup due to the radioactive material. An immunohistochemical assay provides a much simpler and cleaner method for detecting DNA in cells.

Reference Data