BAD pSer136/99 Rabbit Polyclonal Antibody
$330.00
2 Weeks*
Specifications
| Product Data | |
| Application | IHC, WB |
|---|---|
| Recommended Dilution | Immunohistochemistry on paraffin sections: 1:50 - 1:100. Western blot: see ref. 1. |
| Reactivity | Human, Mouse, Rat |
| Antibody Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Synthesized phosphopeptide derived from mouse BAD around the phosphorylation site of serine136 |
| Specificity | BAD (phospho-Ser136) antibody detects endogenous levels of BAD only when phosphorylated at Serine 136. |
| Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol State: Aff - Purified State: Liquid Ig fraction |
| Concentration | lot specific |
| Purification | Affinity chromatography using epitope-specific phosphopeptide |
| Conjugation | Unconjugated |
| Storage | Store the antibody (in aliquots) at -20 °C. Avoid repeated freezing and thawing. |
| Stability | Shelf life: One year from despatch. |
| Shipping | Blue Ice |
| Gene Name | BCL2 associated agonist of cell death |
| Database Link | |
| Background | Bad is a member of the Bcl2 family and acts to promote apoptosis by forming heterodimers with the survival proteins Bcl2 and BclxL, thus preventing them from binding with BAX. Bad is found on the outer mitochondrial membrane and, once phosphorylated in response to growth stimuli, translocates to the cytoplasm. The phosphorylation status of Bad represents a key checkpoint for death or cell survival. JNK-induced phosphorylation of BAD serine 128 promotes the apoptotic role of Bad by opposing the inhibitory effect of growth factor on Bad-mediated apoptosis. Cdc2-induced phosphorylation of Bad serine 128 has an inhibitory effect on its interaction with 14-3-3 proteins. The latter interaction is critical for Bad phosphorylation at serine 155, a site within the BH3 domain that leads to the release of BclxL and the promotion of cell survival. Alternative splicing of this gene results in two transcript variants which encode the same isoform. |
| Synonyms | BAD, BBC6, BCL2L8, Bcl-2-like protein 8, Bcl2-L-8 |
| Reference Data | |
| Protein Categories | Cytokines, Intracellular Proteins |
Reviews
Documents
| Product Manuals |
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Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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