DNA PKcs (PRKDC) Rabbit Polyclonal Antibody
Specifications
| Product Data | |
| Application | WB |
|---|---|
| Recommended Dilution | Western blot: 1/500-1/1000. Incubate membrane with diluted antibody in 5% nonfat milk, 1x TBS, 0.1% Tween-20 at 4°C with gentle shaking overnight. |
| Reactivity | Human |
| Antibody Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Peptide sequence around amino acids 2607~2611 (V-E-T-Q-A) derived from Human DNA PK. |
| Specificity | This antibody detects endogenous levels of total DNA PKcs protein. |
| Buffer | PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol. State: Aff - Purified State: Liquid purified Ig fraction |
| Concentration | lot specific |
| Purification | Immunoaffinity Chromatography using epitope-specific peptide. |
| Conjugation | Unconjugated |
| Storage | Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing. |
| Stability | Shelf life: One year from despatch. |
| Shipping | Blue Ice |
| Gene Name | protein kinase, DNA-activated, catalytic polypeptide |
| Database Link | |
| Background | DNA PK is the key component of the non-homologous end-joining (NHEJ) pathway of DSB repair in mammalian cells. DNA PK consists of a heterodimeric DNA-binding subunit (Ku70/80) and an approximately 465 kDa catalytic subunit (DNA PKcs). DNA PKcs is a serine/threonine protein kinase whose activity is greatly stimulated by its recruitment to DNA breaks by the Ku heterodimer. |
| Synonyms | DNPK1, p460, HYRC, HYRC1 |
| Note | Molecular Weight: 450 kDa |
| Reference Data | |
| Protein Categories | Enzyme: Kinases, Immune system diseases, Intracellular Proteins |
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