MARCKS pSer158 Rabbit Polyclonal Antibody

CAT#: AP02531PU-N

MARCKS pSer158 rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 1. Western blot analysis of extract from starved NIH/3T3 cells, using MARCKS antibody (Lane 1 and 2) and MARCKS pSer158 antibody (Lane 3 and 4).

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  Figure 2. Immunofluorescence staining of methanol-fixed HeLa cells using MARCKS pSer158 antibody (Red).

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
  Immunofluorescence: 1/100-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human MARCKS around the phosphorylation site of serine 158 (R-F-SP-F-K).
• Specificity: This antibody detects endogenous levels of MARCKS only when phosphorylated at Serine 158.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: myristoylated alanine rich protein kinase C substrate
• Database Link:
  Entrez Gene 4082 Human
  UniProt ID P29966
• Background: MARCKS, (Myristoylated Alanine-Rich C Kinase Substrate), is a member of a family of calmodulin binding proteins and is a major substrate for phosphorylation by protein kinase C (PKC). The phosphorylation of Ser152/156 can be used as a measure of PKC activation. Phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin.
• Synonyms: Myristoylated alanine-rich C-kinase substrate, MACS, PRKCSL