DOK1 pTyr362 Rabbit Polyclonal Antibody
Specifications
| Product Data | |
| Application | IHC, WB |
|---|---|
| Recommended Dilution | Western Blot: 1/500-1/1000. Immunohistochemistry on Paraffin Sections: 1/50-1/100. |
| Reactivity | Human, Mouse, Rat |
| Antibody Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | The antiserum was produced against synthesized phosphopeptide derived from Human p62Dok around the phosphorylation site of tyrosine 362 (P-I-Yp-D-E). |
| Specificity | This antibody detects endogenous levels of p62Dok only when phosphorylated at Tyrosine 362. |
| Buffer | PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol State: Aff - Purified State: Liquid purified Ig fraction |
| Concentration | lot specific |
| Purification | Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site. |
| Conjugation | Unconjugated |
| Storage | Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Gene Name | docking protein 1 |
| Database Link | |
| Background | DOK1 (Downstream of tyrosine kinase 1, or Docking protein 1) is believed to be a mainly cytoplasmic adaptor protein which down-regulates mitogen-activated protein kinase activation, inhibits cell proliferation and transformation, and promotes cell spreading and cell migration. DOK1 appears to be a negative regulator of the insulin signaling pathway. |
| Synonyms | Docking protein 1, pp62, p62(dok) |
| Note | Molecular Weight: 62 kDa |
| Reference Data | |
| Protein Categories | Intracellular Proteins |
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