ZAP70 pTyr319 Rabbit Polyclonal Antibody
Specifications
| Product Data | |
| Application | IF, WB |
|---|---|
| Recommended Dilution | Western Blot: 1/500 - 1/1000.; Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0,1% Tween-20 at 4°C with gentle shaking, overnight. Immunofluorescence: 1/100 - 1/200. |
| Reactivity | Human, Mouse, Rat |
| Antibody Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Synthetic phosphopeptide derived from human Zap-70 around the phosphorylation site of Tyrosine 319 (S-P-Yp-S-D). |
| Specificity | This antibody detects endogenous levels of VEGFR2 only when phosphorylated at Tyrosine 1214. |
| Buffer | PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol State: Aff - Purified State: Liquid purified Ig fraction |
| Concentration | lot specific |
| Purification | Immunoaffinity Chromatography |
| Conjugation | Unconjugated |
| Storage | Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing. |
| Stability | Shelf life: One year from despatch. |
| Shipping | Blue Ice |
| Predicted Protein Size | 70kd |
| Gene Name | zeta chain of T cell receptor associated protein kinase 70kDa |
| Database Link | |
| Background | Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not. |
| Synonyms | ZAP-70, ZAP 70, SRK |
| Reference Data | |
| Protein Categories | Allergies and autoimmune diseases, Enzyme: Kinases, Intracellular Proteins |
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