CDK1 pThr161 Rabbit Polyclonal Antibody
$330.00
2 Weeks*
Specifications
| Product Data | |
| Application | IHC, WB |
|---|---|
| Recommended Dilution | Suitable for use in Western blot (1:500~1:1000) and Immunohistochemistry (1:50~1:100). |
| Reactivity | Human, Mouse |
| Antibody Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | The antiserum was produced against synthesized phosphopeptide derived from human CDC2 around the phosphorylation site of threonine161 (T-Y-TP-H-E). |
| Specificity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. CDC2 (phospho-Thr161) antibody detects endogenous levels of CDC2 only when phosphorylated at threonine 161. |
| Buffer | PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol. State: Aff - Purified State: Liquid purified Ig fraction. |
| Concentration | lot specific |
| Purification | Immunoaffinity chromatography. |
| Conjugation | Unconjugated |
| Storage | Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing. |
| Stability | Shelf life: One year from despatch. |
| Shipping | Blue Ice |
| Gene Name | cyclin-dependent kinase 1 |
| Database Link | |
| Background | The cell division control protein cdc2, also known as cyclin dependent kinase 1 (Cdk1) or p34/cdk1, plays a key role in the control of the eukaryotic cell cycle, where it is required for entry into S phase and mitosis. Cdc2 exists as a complex with both cyclin A and cyclin B. The best characterized of these associations is the Cdc2 p34 cyclin B complex, which is required for the G2 to M phase transition. Activation of Cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161. However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Tyr14. Phosphorylation at Tyr15 and inhibition of Cdc2 is carried out by WEE1 and MIK protein kinases while Tyr15 dephosphorylation and activation of Cdc2 is carried out by the cdc25 phosphatase. The isoform CDC2deltaT is found in breast cancer tissues. Furthermore, cdc2/Cdk1 is a key mediator of neuronal cell death in brain development and degeneration. |
| Synonyms | CDK1, CDC2, CDC28A, CDKN1, P34CDC2, p34 protein kinase |
| Reference Data | |
| Protein Categories | Enzyme: Kinases, Intracellular Proteins |
Reviews
Documents
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Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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