SUMO-2/3 Mouse Monoclonal Antibody [Clone ID: SM23/496]
$665.00
2 Weeks*
Specifications
| Product Data | |
| Clone Name | SM23/496 |
|---|---|
| Application | FC, IF, IHC, IP, WB |
| Recommended Dilution | ELISA: Use BSA free Antibody for coating. Flow Cytometry: 0.5-1 µg/million cells. Immunofluorescence: 0.5-1 µg/ml. Western Blot: 0.5-1 µg/ml. Immunoprecipitation: 0.5-1 µg/500 µg protein lysate. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Formalin-Fixed Sections: 0.5-1 µg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: HeLa cells or breast carcinoma. |
| Reactivity | Human |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | Recombinant human SUMO2 protein |
| Specificity | Cellular Localization: Nucleus, PML body |
| Buffer | 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
| Concentration | lot specific |
| Purification | Protein A/G Chromatography |
| Conjugation | Unconjugated |
| Storage | Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Predicted Protein Size | 11-13kDa |
| Database Link | |
| Background | This MAb reacts with both SUMO-2 and SUMO-3. The small ubiquitin-related modifier (SUMO) proteins, which include SUMO-1, 2 and 3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Also, both utilize the E1, E2 and E3 cascade enzymes for conjugation. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO-1, 2 and 3 proteins localize to the nuclear membrane, nuclear bodies and cytoplasm, respectively. SUMO-1 utilizes Ubc9 for conjugation to several target proteins, which include MDM2, p53, PML and RanGap1. SUMO-2 and 3 contribute to a greater percentage of protein modification than does SUMO-1. In addition, SUMO-3 regulates beta-Amyloid generation and may be critical in the onset or progression of Alzheimer's disease. |
| Synonyms | HSMT3; Sentrin 2; Small ubiquitin like modifier 2; Small ubiquitin like modifier protein 3; SMT3 homolog; SMT3 homolog 1 (SMT3H1); SMT3 homolog 2 (SMT3H2); SMT3 suppressor of mif two 3 homolog 1; SMT3 suppressor of mif two 3 homolog 2; SMT3 suppressor of mif two 3 homolog 3; SMT3A; SMT3B; Suppressor of mif two 3 homolog 2; Suppressor of mif two 3 homolog 3; Ubiquitin like protein SMT3A; Ubiquitin like protein SMT3B |
| Note | Hu Chromosome Location: 17q25.1 (SUMO-2) & 21q22.3 (SUMO-3) |
| Reference Data | |
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Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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