MUC5AC (Mucin 5AC / Gastric Mucin) Mouse Monoclonal Antibody [Clone ID: SPM297]
Specifications
| Product Data | |
| Clone Name | SPM297 |
|---|---|
| Application | FC, IF, IHC, IP, WB |
| Recommended Dilution | ELISA: Use BSA free Antibody for coating. Flow Cytometry: 0.5-1 μg/million cells. Immunofluorescence: 1-2 μg/ml. Western Blotting: 0.5-1 μg/ml. Immunoprecipitation: 1-2 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-fixed Sections: 0.5-1.0 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: Stomach. |
| Reactivity | Chicken, Feline, Human, Monkey, Mouse, Pig, Porcine, Rabbit, Rat |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient |
| Specificity | Cellular Localization: Secreted |
| Buffer | 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
| Concentration | lot specific |
| Purification | Protein A/G Chromatography |
| Conjugation | Unconjugated |
| Storage | Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Predicted Protein Size | >1|000kDa |
| Gene Name | mucin 5AC, oligomeric mucus/gel-forming |
| Database Link | |
| Background | This MAb recognizes the peptide core of gastric mucin M1 (1,000kDa) (recently identified as Mucin 5AC). Its epitope is destroyed by beta-mercaptoethanol and proteases but not by periodate treatment. Antibody to gastric mucin M1 reacts with the gastric epithelium of normal human gastrointestinal tract as well as with the precancerous and cancerous colon but not with normal adult colon. It also reacts with fetal colonic mucosa. Resurgence of gastric mucin reactivity during colonic carcinogenesis is due to re-expression of the peptide core of gastric (or fetal colonic) mucins. |
| Synonyms | Apomucin Major Airway Glycoprotein, Mucin 5 subtype AC tracheobronchial, Mucin 5 Subtypes A And C, Mucin 5AC oligomeric mucus/gel forming, Tracheobronchial Mucin (TBM) |
| Reference Data | |
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