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MUC5AC (Mucin 5AC / Gastric Mucin) Mouse Monoclonal Antibody [Clone ID: SPM297]

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SKU
AM50143PU-S
MUC5AC (Mucin 5AC / Gastric Mucin) mouse monoclonal antibody, clone SPM297, Purified
Size:
100 ug 20 ug
Conjugation:
Unconjugated
  $665.00
2 Weeks*
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View other "MUC5AC" antibodies

Specifications
Specifications
Product Data
Clone Name SPM297
Application FC, IF, IHC, IP, WB
Recommended Dilution ELISA: Use BSA free Antibody for coating.
Flow Cytometry: 0.5-1 μg/million cells.
Immunofluorescence: 1-2 μg/ml.
Western Blotting: 0.5-1 μg/ml.
Immunoprecipitation: 1-2 μg/500 μg protein lysate.
Immunohistochemistry on Frozen and Formalin-fixed Sections: 0.5-1.0 μg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Positive Control: Stomach.
Reactivity Chicken, Feline, Human, Monkey, Mouse, Pig, Porcine, Rabbit, Rat
Antibody Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient
Specificity Cellular Localization: Secreted
Buffer 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration lot specific
Purification Protein A/G Chromatography
Conjugation Unconjugated
Storage Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Predicted Protein Size >1|000kDa
Gene Name mucin 5AC, oligomeric mucus/gel-forming
Database Link
Background This MAb recognizes the peptide core of gastric mucin M1 (1,000kDa) (recently identified as Mucin 5AC). Its epitope is destroyed by beta-mercaptoethanol and proteases but not by periodate treatment. Antibody to gastric mucin M1 reacts with the gastric epithelium of normal human gastrointestinal tract as well as with the precancerous and cancerous colon but not with normal adult colon. It also reacts with fetal colonic mucosa. Resurgence of gastric mucin reactivity during colonic carcinogenesis is due to re-expression of the peptide core of gastric (or fetal colonic) mucins.
Synonyms Apomucin Major Airway Glycoprotein, Mucin 5 subtype AC tracheobronchial, Mucin 5 Subtypes A And C, Mucin 5AC oligomeric mucus/gel forming, Tracheobronchial Mucin (TBM)
Reference Data
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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