OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Cdc20 (Cell Division Cycle Protein 20) Mouse Monoclonal Antibody [Clone ID: AR12]

Be the first to review this product
SKU
AM33366PU-T
Cdc20 (Cell Division Cycle Protein 20) mouse monoclonal antibody, clone AR12, Purified
Size:
20 ug 100 ug
Conjugation:
Unconjugated
  $370.00
2 Weeks*
Bulk/Customize

View other "CDC20 or NP_001246.2" antibodies

Specifications
Specifications
Product Data
Clone Name AR12
Application FC, IF, IHC, IP, WB
Recommended Dilution ELISA: Use BSA free Antibody for coating.
Flow Cytometry: 0.5-1 μg/million cells.
Immunofluorescence: 0.5-1 μg/ml.
Western Blotting: 0.5-1 μg/ml.
Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Positive Control: Ramos or HeLa cells; Tonsil.
Reactivity Human
Antibody Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Urea-denatured His6 Cdc20 human recombinant protein
Specificity Cellular Localization: Centrosome, Cytoplasm, Cytoskeleton, Microtubule organizing center, Spindle pole
Buffer 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration lot specific
Purification Protein A/G Chromatography
Conjugation Unconjugated
Storage Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Predicted Protein Size 55kDa
Gene Name cell division cycle 20
Database Link
Background Cyclins, regulatory subunits, which associate with kinases, control many of the important steps in cell cycle progression. The Cdc2 protein kinase (p34Cdc2) exhibits protein kinase activity in vitro and exists in a complex with both cyclin B and a protein homologous to p13SUC1. Cdc2 kinase is the active subunit of the M phase promoting factor (MPF) and the M phase-specific Histone H1 kinase. The p34Cdc2/cyclin B complex is required for the G2 to M transition. An additional cell cycle-dependent protein kinase, termed p55cdc, exhibits a high degree of homology with the S. cerevisiae proteins Cdc20 and Cdc4. The p55cdc transcript is readily detectable in a variety of cultured cell lines in growth phase, but disappears when cell growth is chemically arrested.
Synonyms CDC20, CDC20A, p55CDC, P55CDC-LSB
Reference Data
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products