CDC20 Mouse Monoclonal Antibody [Clone ID: AR12]

SKU AM33366PU-S

CDC20 mouse monoclonal antibody, clone AR12, Purified

Product Data

• Clone Name: AR12
• Application: FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: Use BSA free Antibody for coating.; Flow Cytometry: 0.5-1 μg/million cells.; Immunofluorescence: 0.5-1 μg/ml.; Western Blotting: 0.5-1 μg/ml.; Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.; Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT.; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.; Positive Control: Ramos or HeLa cells; Tonsil.
• Reactivity: Human
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Urea-denatured His6 Cdc20 Human recombinant protein.
• Specificity: This antibody reacts with Human Cdc20 (55 kDa). Other species not tested. ; Cellular Localization: Cytoplasmic.
• Buffer: 10mM PBS; State: Purified; State: Liquid purified IgG fraction from Bioreactor Concentrate; Stabilizer: 0.05% BSA; Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Predicted Protein Size: 55 kDa
• Gene Name: cell division cycle 20
• Database Link:
  Q12834
• Background: Cyclins, regulatory subunits which associate with kinases, control many of the important steps in cell cycle progression. The Cdc2 protein kinase (p34Cdc2) exhibits protein kinase activity in vitro and exists in a complex with both cyclin B and a protein homologous to p13SUC1. Cdc2 kinase is the active subunit of the M phase promoting factor (MPF) and the M phase-specific Histone H1 kinase. The p34Cdc2/cyclin B complex is required for the G2 to M transition. An additional cell cycle-dependent protein kinase, termed p55cdc, exhibits a high degree of homology with the S. cerevisiae proteins Cdc20 and Cdc4. The p55cdc transcript is readily detectable in a variety of cultured cell lines in growth phase, but disappears when cell growth is chemically arrested.
• Synonyms: p55CDC

Reference Data