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Actin, Muscle Specific (Muscle Cell Marker) Mouse Monoclonal Antibody [Clone ID: SPM160]

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SKU
AM33291PU-T
Actin, Muscle Specific (Muscle Cell Marker) mouse monoclonal antibody, clone SPM160, Purified
Size:
20 ug 100 ug
Conjugation:
Unconjugated
  $370.00
5 Days*
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View other "ACTA2 or NP_001091.1" antibodies

Specifications
Specifications
Product Data
Clone Name SPM160
Application FC, IF, IHC, IP, WB
Recommended Dilution Western Blotting: 0.5-1 µg/ml.
Flow Cytometry: 0.5-1 µg/106 cells.
Immunoprecipitation: 0.5-1 µg/500 µg protein lysate.
Immunofluorescence: 0.5-1 µg/ml.
Immunohistochemistry on Frozen and Formalin-Fixed Sections: 0.5-1 µg/ml for 30 minutes at RT.
No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
Recommended Positive Control: Muscle or sarcoma.
Reactivity Human, Rabbit, Rat
Antibody Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen SDS extract of human myocardium.
Specificity Cellular Localization: Cytoplasm, Cytoskeleton
Buffer 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration lot specific
Purification Protein A/G Chromatography
Conjugation Unconjugated
Storage Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Predicted Protein Size Multiple
Database Link
Background This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotype of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
Synonyms ACTA, ACTA1, ACTA2, ACTC1, Actin, ACTSA, Alpha-2 actin, alpha skeletal muscle, Alpha-actin-1, Cardiac muscle alpha actin-1, Skeletal muscle alpha actin-1
Reference Data
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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