RPP20 (POP7) Mouse Monoclonal Antibody [Clone ID: 1F11]

CAT#: AM32014PU-N

RPP20 (POP7) mouse monoclonal antibody, clone 1F11, Purified

Product Images

Figure 1. Specificity of anti-hRPP20 Immunoglobulin, clone 1F11, determined by immunoblot analysis Blot contains total cell extract of HEp2 cells. Incubated with antibody fraction (0.5 mg/ml) diluted 500X in PBS containing 0.05% tween-20 and 5% non fat dry milk. Left: washing under stringent conditions. Right: washing under less stringent conditions.

Specifications

Product Data

• Clone Name: 1F11
• Applications: ELISA, WB
• Recommended Dilution: ELISA.
  Immunoblotting.
  Recommended Solvent: 100 mM PBS or Tris-HCl, pH 7.0
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Recombinant GST-hRPP20 (NCBI accession number AAC24113, expression vector pGEC-2T), expressed in E.coli.
• Specificity:

  Specificity has been tested in ELISA.
  In immunoblotting, under less stringent washing conditions, a weaker band of approximately 60 kDa is also detected (Figure 1, Right Panel).
  Additional tests for cross reactivity have not yet been performed.

• Formulation: 10 mM Ammonium Bicarbonate buffer
  State: Purified
  State: Lyophilized purified Ig fraction
  Stabilizer: 2 mg BSA
• Reconstitution Method: Dissolve in 200 μl buffer for a 0.5 mg/ml antibody concentration in 1% BSA
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C or Add up to 0.05% Sodium Azide for long term storage.
  
• Stability: Shelf life: One year from despatch.
• Predicted Protein Size: 20 kDa
• Gene Name: POP7 homolog, ribonuclease P/MRP subunit
• Database Link:
  Entrez Gene 10248 Human
  UniProt ID O75817
• Background:

  The nuclei of eukaryotic cells contain several classes of small RNA-protein complexes. SnRNPs (small nuclear ribonucleoproteins) are involved in splicing of pre-mRNAs (the excision of introns) in the nucleoplasm. RPP20 is part of such a complex. SnoRNPs (small nucleolar ribonucleoproteins) are involved in the maturation of pre-ribosomal RNA in the nucleoli.
  Pre-rRNA processing and modification require both the snoRNA and the protein constituents (such as Rpp20) of these complexes. Generally, snoRNPs contain a number of common proteins, which are shared with other snoRNPs of the same family, next to several particle-specific proteins. On average, snoRNPs contain about 6-10 protein subunits.

• Synonyms: hPOP7, RPP20