RPP20 (POP7) Mouse Monoclonal Antibody [Clone ID: 1F11]

SKU AM32014PU-N

RPP20 (POP7) mouse monoclonal antibody, clone 1F11, Purified

Product Data

• Clone Name: 1F11
• Application: ELISA, WB
• Recommended Dilution: ELISA. ; Immunoblotting. ; Recommended Solvent: 100 mM PBS or Tris-HCl, pH 7.0
• Reactivity: Human
• Antibody Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Recombinant GST-hRPP20 (NCBI accession number AAC24113, expression vector pGEC-2T), expressed in E.coli.
• Specificity:

  Specificity has been tested in ELISA.; In immunoblotting, under less stringent washing conditions, a weaker band of approximately 60 kDa is also detected (Figure 1, Right Panel). ; Additional tests for cross reactivity have not yet been performed.

• Buffer: 10 mM Ammonium Bicarbonate buffer; State: Purified; State: Lyophilized purified Ig fraction; Stabilizer: 2 mg BSA
• Reconstitution Method: Dissolve in 200 μl buffer for a 0.5 mg/ml antibody concentration in 1% BSA
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C or Add up to 0.05% Sodium Azide for long term storage.;
• Stability: Shelf life: One year from despatch.
• Shipping: Ambient
• Predicted Protein Size: 20 kDa
• Gene Name: POP7 homolog, ribonuclease P/MRP subunit
• Database Link:
  O75817
• Background:

  The nuclei of eukaryotic cells contain several classes of small RNA-protein complexes. SnRNPs (small nuclear ribonucleoproteins) are involved in splicing of pre-mRNAs (the excision of introns) in the nucleoplasm. RPP20 is part of such a complex. SnoRNPs (small nucleolar ribonucleoproteins) are involved in the maturation of pre-ribosomal RNA in the nucleoli. ; Pre-rRNA processing and modification require both the snoRNA and the protein constituents (such as Rpp20) of these complexes. Generally, snoRNPs contain a number of common proteins, which are shared with other snoRNPs of the same family, next to several particle-specific proteins. On average, snoRNPs contain about 6-10 protein subunits.

• Synonyms: hPOP7, RPP20

Reference Data