MHC Class I H-2 Kk Mouse Monoclonal Antibody [Clone ID: H100-5/28]
Specifications
| Product Data | |
| Clone Name | H100-5/28 |
|---|---|
| Application | FC |
| Recommended Dilution | Flow Cytometry. Test Results: Mouse Strain: CBA Cell Concentration : 1x10e6 cells per test. Antibody Concentration Used: 0.5 μg/10e6 cells. Isotypic Control: PE Mouse IgG2a, Cell Source Percentage of cells stained above control: Spleen: 97.3% |
| Reactivity | Mouse |
| Antibody Host | Mouse |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Specificity | This Monoclonal Antibody specifically reacts with the H-2Kk (Determinant H-2.m11) encoded MHC Class I antigen expressed on mouse strains of the H-2k haplotype. This antibody is also reported to react with H-2Kq and H-2r haplotypes. Class I antigens are expressed on all nucleated cells. |
| Buffer | PBS containing 0.02% Sodium Azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml Label: PE State: Liquid purified Ig fraction |
| Concentration | lot specific |
| Purification | Protein G Chromatography |
| Conjugation | PE |
| Storage | Store the antibody undiluted at 2-8°C . DO NOT FREEZE! Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Database Link | |
| Background | MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta2- microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. Lymphocytes are highly positive whereas fibroblasts or neurons show only a low level of antigen. |
| Synonyms | H2-K1, H2-K, H-2K(K) |
| Reference Data | |
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