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CD45 / LCA (CD45RC) Rat Monoclonal Antibody [Clone ID: IBL-8]

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SKU
AM31873RP-L
CD45 / LCA (CD45RC) rat monoclonal antibody, clone IBL-8, PE
Size:
300 ug
Conjugation:
PE Biotin FITC
  $1,205.00
2 Weeks*
Bulk/Customize

View other "Ptprc" antibodies

Specifications
Specifications
Product Data
Clone Name IBL-8
Application FC
Recommended Dilution Immunohistochemistry on acetone-fixed frozen sections.
Flow Cytometry.
Immunoprecipitation.
Reactivity Mouse
Antibody Host Rat
Isotype IgG1
Clonality Monoclonal
Immunogen Mouse Spleen Cells
Donor: Wistar spleen
Fusion Partner: Sp-2/0 Ag14
Specificity Anti-mouse CD45RC is against the exon C-dependent RC isoform and reacts strongly with B cells, and less intensely with most CD8+ T cells. It does not recognize CD4+ T cells. Also, myeloid cells do not express the RC isoform.
Buffer PBS containing 0.02% sodium azide (NaN3) as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.
Label: PE
State: Liquid purified Ig fraction
Label: R - Phycoerythrin
Concentration lot specific
Purification Affinity purified
Conjugation PE
Storage Store the antibody undiluted at 2-8°C.
DO NOT FREEZE!
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Gene Name protein tyrosine phosphatase, receptor type, C
Database Link
Background CD45 (L-CA) is a transmembrane phosphotyrosine phosphatase expressed on leukocytes.
Synonyms PTPRC, Leukocyte common antigen, L-CA, T200
Note Protocol: FLOW CYTOMETRY ANALYSIS:
Method:
1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-M cell separation medium.
2. Wash 2 times.
3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 μl of this suspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test).
4. To each tube, add 1.0 μg of this antibody per 10e6 cells.
5. Vortex the tubes to ensure thorough mixing of antibody and cells.
6. Incubate the tubes for 30 minutes at 4°C.
(It is recommended that the tubes are protected from light, since most fluorochromes are light sensitive.)
7. Wash 2 times at 4°C.
8. Resuspend the cell pellet in 50 μl ice cold media B.
9. Transfer to suitable tubes for flow cytometric analysis containing 15 μl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA.
Media:
A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 μl of 2M sodium azide in 100 mls).
B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 μl of 2M sodium azide in 100 mls).
Results:
Tissue Distribution by Flow Cytometry Analysis:
Mouse Strain: BALB/c
Cell Concentration: 1x10e6 cells per test
Antibody Concentration Used: 1.0 μg/10e6 cells
Isotypic Control: PE Rat IgG1
Reference Data
Protein Categories ADC Targets, CD Markers, Cytokines, Growth Factors, Immune system diseases, Intracellular Proteins, Membrane Proteins
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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