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BPDE-DNA Mouse Monoclonal Antibody [Clone ID: 5D11]

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SKU
AM26349PU-N
BPDE-DNA mouse monoclonal antibody, clone 5D11, Purified
Size:
100 ug
Conjugation:
Unconjugated
  $840.00
2 Weeks*
Bulk/Customize
Specifications
Specifications
Product Data
Clone Name 5D11
Application ELISA, IF, IHC, IP
Recommended Dilution Flow cytometry (8): Washed sperm was fixed in 2% paraformaldehyde and permeabilized with 0.2% triton x-100/0.1% sodium citrate. Samples were treated with protK and RNase. To denature DNA samples were incubated with 4n HCl. After blocking with 5% normal serum samples were incubated with mAb.
Immunoassays (4,7): Plates were coated with 50 ng/well BPDE-DNA in 50mM Tris-buffer pH7.5 o/n at 4°C. Plates were blocked 1% FCS. DNA samples, 4µg, were mixed with 5D11 and added to the well. Detection with GtαMs-IgG-AP for 90’at 37°C.
Immunoflourescence (8).
Immunoprecipitation (10).
Immunohistochemistry on paraffin sections (2,3,5,6,9): 5 µm sections were RNase and prot-K treated. DNA was denatured with 4N HCl and neutralized with 50mM Tris base. Section was blocked with 1.5% normal horse serum: The typical starting working dilution is 1:10.
Antibody Host Mouse
Isotype IgG2a
Clonality Monoclonal
Immunogen BPDE-I-DNA complexed with methylated BSA
Specificity The monoclonal antibody 5D11 recognizes BPDE-I-DNA (PAH-DNA).
Buffer PBS
State: Purified
State: Liquid 0.2 µm filtered Ig fraction
Stabilizer: 0.1% bovine serum albumin
Preservative: 0.02% sodium azide
Concentration lot specific
Purification Protein G
Conjugation Unconjugated
Storage

Store at 2 - 8 °C.
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Background

A number of chemicals, including polycyclic aromatic hydrocarbons (PAHs), have been shown to bind to DNA. This DNA damage can occur both early and late in the malignant process, thereby acting as an initiator and assisting in the progression of tumors. PAHs are released into the environment following incomplete combustion of organic materials. The most common sources of PAHs are from smoking and from consuming broiled or grilled foods. Human exposure to PAHs comes from various occupational, environmental, dietary and medicinal sources. Benzoapyrene is a representative PAH. Antibodies to benzoapyrenediol-epoxide modified DNA (BPDE-DNA) can be used to identify polycyclic aromatic hydrocarbon (PAH)-DNA adducts. Exposure to this group of compounds is believed to be carcinogenic.
Reference Data
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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