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BPV-1 E2 tag (SSTSSDFRDR) Mouse Monoclonal Antibody [Clone ID: 5E11]

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SKU
AM26099PU-N
BPV-1 E2 tag (SSTSSDFRDR) mouse monoclonal antibody, clone 5E11, Aff - Purified
Size:
100 ug
Conjugation:
Unconjugated
  $595.00
2 Weeks*
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View other "E2" antibodies

Specifications
Specifications
Product Data
Clone Name 5E11
Application IF, IP, WB
Recommended Dilution Western blot (1/1000–1/5000): Dilute to a concentration of 1.0-0.2 µg/ml in a diluent containing 2% non-fat dry milk in TBST (Tris-Buffered Saline, Tween-20) (Background can be reduced by adding NaCl up to a concentration of 1.5 M). Incubate the membrane with diluted anti-E2Tag antibody for 1 hour at room temperature with gentle orbital shaking.
Immunofluorescence (1/1000): Dilute anti-E2Tag antibody to a concentration of 1-2 µg/ml in a diluent containing 1% BSA in PBS. Incubate with diluted anti-E2Tag antibody for 1 hour at room temperature.
Immunoprecipitation.
This antibody belongs to the IgG1 subclass, thus, we recommend using protein G-Agarose for Binding experiments.
Reactivity Bovine
Antibody Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Recombinant full length BPV type 1 E2 protein purified from E. coli
Specificity This antibody detects Bovine Papillomavirus E2-protein mapped to aa 199-208 (E2Tag, SSTSSDFRDR).
Isolates E2 Tag-tagged proteins and associated proteins/protein binding partners by Immunoprecipitation.
Buffer PBS with 50% Glycerol
State: Aff - Purified
State: Liquid purified Ig fraction
Concentration lot specific
Purification Affinity Chromatography on Protein A
Conjugation Unconjugated
Storage Upon receipt, store (in aliquots) at -20°C to -70 °C.
Avoid repeated freezing and thawing.
Stability Shelf life: One year from despatch.
Shipping Blue Ice
Database Link
Background The bovine papilloma virus E2 protein is the master regulator of papillomavirus replication and transcription. Binds to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory region. Can either activate or repress transcription depending on E2RE's position with regards to proximal promoter elements. Repression occurs by sterically hindering the assembly of the transcription initiation complex. The E1-E2 complex binds to the origin of DNA replication.
Synonyms BPV1, Bovine Papilloma Virus Protein E2
Reference Data
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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