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ERK1 / ERK2 Mouse Monoclonal Antibody [Clone ID: IL-13]

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SKU
AM20636PU-N
ERK1 / ERK2 mouse monoclonal antibody, clone IL-13, Purified
Size:
100 ug
Conjugation:
Unconjugated
  $600.00
2 Weeks*
Bulk/Customize
Specifications
Specifications
Product Data
Clone Name IL-13
Application IF, IHC, WB
Recommended Dilution Western Blot: 0.25 - 0.5 μg/ml.
Immunohistochemistry on paraffin sections: 0.4 - 1 μg/ml.
Immunocytochemistry.
Reactivity Human, Insect, Mouse, Rat, Yeast
Antibody Host Mouse
Isotype IgG1
Clonality Monoclonal
Specificity This antibody reacts to ERK1 / ERK2.
Buffer 1.2 % sodium acetate, with 2 mg BSA and 0.01 mg sodium azide as preservative.
State: Purified
State: Lyphilized purified Ig fraction
Reconstitution Method Restore with 1.2% sodium acetate or neutral PBS
Concentration 0,1 mg/ml (after reconstitution with PBS)
Purification Affinity chromatography
Conjugation Unconjugated
Storage Prior to reconstitution store at -20°C.
Following reconstitution store undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Shipping Ambient
Background In mammalian cells, a variety of extracellular stimuli generate intracellular signals that converge on a limited number of so-called mitogen-activated protein (MAP) kinase pathways. The central core of each MAP kinase (MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase (MAPKKK) phosphorylates and activates a specific MAPK kinase (MAPKK), which then activates a specific MAPK. Mek1/2 MAPK kinases are essential for mammalian development, homeostasis, and Raf-induced hyperplasia. Germline mutations in genes within the MAPK pathway cause cardio-facio-cutaneous syndrome.
Synonyms ERK-1/ERK-2, MAPK1/MAPK2, P42/P44-MAPK
Reference Data
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Documents
Resources

file-alt Citations

A robust and economical pulse-chase protocol to measure the turnover of HaloTag fusion proteins
Merrill, Song, Kephart et al
J. Biol. Chem. (2019)
AI Summary: OriGene’s mouse anti-DDK (FLAG) antibody (Cat# TA50011) was used in this study to detect DDK-tagged (FLAG-tagged) proteins, facilitating the analysis of HaloTag fusion protein turnover in their pulse-chase experiments.
Snippet: The following commercially-sourced antibodies and labels were used: rabbit anti-GFP (Abcam, Cat# ab290), rabbit anti-B'β serum (14), mouse anti-DDK (FLAG) (OriGene, Cat# TA50011), rabbit anti-ERK1/2 (SCBT, Cat# sc94), mouse anti-doublecortin (Sant Cruz, Cat
WB
The protective effect of eupafolin against TNF-α-induced lung inflammation via the reduction of intercellular cell adhesion molecule-1 expression
Sung, Liang, Lee et al
J Ethnopharmacol (2015)
Cited by 29
AI Summary: OriGene's anti-total JNK antibody was used in this study to assess JNK protein levels by Western blotting, contributing to the analysis of signaling pathways involved in TNF-α-induced lung inflammation.
Snippet: phospho AKT (1:1000, EnoGene Biotech), anti-phospho p65 (1:1000, Epitomics, Burlingame, CA, USA), anti-phospho IκB (1:1000, Cell Signaling), anti-total p65 (1:1000, Epitomics), anti-total ERK1/2 (1:10000, Cell signaling), and anti-total JNK (1:1000, OriGene, Rockville ... Cite SaveSaving
WB IHC IF
Showing 1-2 of 2 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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