GRP94 (HSP90B1) (676-803) Mouse Monoclonal Antibody [Clone ID: 2H3]

CAT#: AM09037PU-N

GRP94 (HSP90B1) (676-803) mouse monoclonal antibody, clone 2H3, Purified

Product Images

Western blot analysis: The cell lysates of hES (20 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with HSP90B1 antibody (1/500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Immunohistochemistry: HSP90B1 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Breast at 5 ug/ml followed by biotinylated anti-Mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and chromogen.

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  Immunohistochemistry: HSP90B1 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Heart at 5 ug/ml followed by biotinylated anti-Mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and chromogen.

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  Immunofluorescence staining of human ES cell colony with monoclonal anti-human TRA1 antibody (2H3).

Specifications

Product Data

• Clone Name: 2H3
• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA.
  Western blot.
  Immunofluorescence (1/100-1/200).
  Immunohistochemistry on Paraffin Sections (5 µg/ml).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Recombinant Human TRA1 (676-803 aa) purified from E. coli
• Specificity: The antibody recognizes Human TRA1. Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: heat shock protein 90kDa beta family member 1
• Database Link:
  Entrez Gene 7184 Human
  UniProt ID P14625
• Background: Tumor rejection antigen 1 (TRA1) is expressed on the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). TRA1 is a marker protein of these stem cells.
• Synonyms: Tumor rejection antigen 1, GRP94